LOCUS Exported 4983 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBabe-HAZ SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4983) AUTHORS Gao G, Guo X, Goff SP TITLE Inhibition of retroviral RNA production by ZAP, a CCCH-type zinc finger protein. JOURNAL Science. 2002 Sep 6. 297(5587):1703-6. PUBMED 12215647 REFERENCE 2 (bases 1 to 4983) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4983 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 8..477 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 542..741 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 934..1350 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1318..1334 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" primer_bind complement(1411..1431) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 1416..1745 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 1596..1731 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1658..1677 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 1752..1761 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1805..2176 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="AKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDV TLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGRE FALRDPAGNCVHFVAEEQD" protein_bind complement(2257..2290) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 2382..2851 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(3054..3071) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3225..3813) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3305..3324) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3995..4855) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4618..4637 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4856..4960) /gene="bla" /label=AmpR promoter ORIGIN 1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt 961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc 1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg 1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca 1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct 1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt 1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt 1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga 1381 attaattcgc cagcacagtg gtcgacctgt ggaatgtgtg tcagttaggg tgtggaaagt 1441 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 1501 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 1561 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 1621 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 1681 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 1741 gcaaaaagct tgccaccatg gcttatccat atgatgttcc agatatgctg aattcggcgg 1801 ccgcgccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt 1861 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg 1921 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga 1981 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga 2041 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca 2101 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc 2161 cgaggagcag gactgaatcg ataaaataaa agattttatt tagtctccag aaaaaggggg 2221 gaatgaaaga ccccacctgt aggtttggca agctagataa cttcgtataa tgtatgctat 2281 acgaagttat ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag 2341 aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag 2401 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga 2461 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac 2521 agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc 2581 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc 2641 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct 2701 cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa 2761 ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag 2821 tgattgacta cccgtcagcg ggggtctttc atttccgact tgtggtctcg ctgccttggg 2881 agggtctcct ctgagtgatt gactacccgt cagcgggggt cttcacatgc agcatgtatc 2941 aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt tgcattaatg 3001 aatcggccaa cgcgcgggga gaggcggttt gcgtattggc gctcttccgc ttcctcgctc 3061 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 3121 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 3181 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 3241 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 3301 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 3361 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 3421 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 3481 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 3541 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 3601 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 3661 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 3721 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 3781 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 3841 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 3901 aggatcttca cctagatcct tttaaattaa aaatgaagtt tgcggccggc cgcaaatcaa 3961 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 4021 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 4081 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 4141 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 4201 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 4261 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 4321 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 4381 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 4441 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 4501 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 4561 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata 4621 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 4681 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 4741 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 4801 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 4861 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 4921 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 4981 cac //