LOCUS Exported 1162 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION Localization: Nucleus/Histones, Excitation: 433, Emission: 475. ACCESSION . VERSION . KEYWORDS mCerulean-H4-23 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1162) TITLE Davidson Cyan Fluorescent Proteins REFERENCE 2 (bases 1 to 1162) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1162 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 337..389 /label=MCS /note="multiple cloning site" regulatory 397..406 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 403..1122 /codon_start=1 /product="enhanced monomeric variant of CFP (Rizzo et al., 2004)" /label=mCerulean /note=" mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(448..469) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(709..728) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1056..1077 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 acgtcagatc cgctagccgc caccatgtct ggccgcggca aaggcgggaa gggtcttggc 61 aaaggcggcg ctaagcgcca ccgtaaagta ctgcgcgaca atatccaggg catcaccaag 121 ccggccatcc ggcgccttgc tcgccgcggc ggcgtgaagc gcatctccgg cctcatctac 181 gaggagactc gcggggtgct gaaggtgttc ctggagaacg tgatccggga cgccgtgacc 241 tatacagagc acgccaagcg caagacggtc accgccatgg atgtggtcta cgcgctcaag 301 cgccagggcc gcaccctcta cggtttcggt ggggatctcg agctcaagct tcgaattctg 361 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag 421 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 481 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 541 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc 601 tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag 661 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 721 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 781 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacgcc 841 atcagcgaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc 901 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 961 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc 1021 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 1081 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga 1141 tcataatcag ccataccaca tt //