LOCUS       Exported                1191 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Localization: Mitochondria, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    mCerulean-Mito-7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1191)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        77..128
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     79..99
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             157..231
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      259..268
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             265..984
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(310..331)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(571..590)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     918..939
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1106..1186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccatgacgt caatgggagt tgtttggcac caaaatcaac ggactttcca aaatgtcgta
       61 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      121 gcagagctgg tttagtgaac cgtcagatcc gctagcatgt ccgtcctgac gccgctgctg
      181 ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc cgcgcgccaa gatccattcg
      241 ttgggggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
      301 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      361 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      421 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc
      481 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      541 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      601 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      661 gaggacggca acatcctggg gcacaagctg gagtacaacg ccatcagcga caacgtctat
      721 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
      781 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      841 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
      901 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      961 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
     1021 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     1081 ataaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     1141 aagcaatagc atcacaaatt cacaattaaa gcattttttt cactgcatct a
//
