LOCUS Exported 2083 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION Localization: Lysosome Membrane, Excitation: 433, Emission: 475. ACCESSION . VERSION . KEYWORDS mCerulean3-Lysosomes-20 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2083) AUTHORS Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, Day RN, Wachter RM, Davidson MW, Rizzo MA TITLE An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching. JOURNAL PLoS One. 2011 . 6(3):e17896. PUBMED 21479270 REFERENCE 2 (bases 1 to 2083) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2083 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 36..45 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 40..49 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 42..1262 /codon_start=1 /product="rat lysosome-associated membrane glycoprotein 1" /label=LAMP1 /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR SHAGYQTI" regulatory 1317..1326 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(1368..1389) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1629..1648) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1976..1997 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 agatccgcta gcgctaccgg actcagatct cgagcgccac catggcggcc ccgggcgccc 61 ggcgtccgct gctcctgttg ctgctggcag gccttgcaca cagcgcccca gcactgttcg 121 aggtgaaaga caacaacggc acagcgtgta taatggccag cttctctgcc tcctttctga 181 ccacctatga ggctggacat gtttctaagg tctcgaatat gaccctgcca gcctctgcag 241 aagtcctgaa gaatagcagc tcttgtggtg aaaagaatgc ttctgagccc accctcgcaa 301 tcacctttgg agaaggatat ttactgaaac tcaccttcac aaaaaacaca acacgttaca 361 gtgtccagca catgtatttc acatataacc tgtcagacac acaattcttt cccaatgcca 421 gctccaaagg gcccgacact gtggattcca caactgacat caaggcagac atcaacaaaa 481 cataccgatg tgtcagcgac atcagggtct acatgaagaa tgtgaccatt gtgctctggg 541 acgctactat ccaggcctac ctgccgagta gcaacttcag caaggaagag acacgctgcc 601 cacaggatca accttcccca actactgggc cacccagccc ctcaccacca cttgtgccca 661 caaaccccag tgtgtccaag tacaatgtga ctggtgacaa tggaacctgc ctgctggcct 721 ctatggcact gcaactcaac atcacctaca tgaagaagga caacacgact gtgaccagag 781 cattcaacat caacccaagt gacaaatata gtgggacttg cggtgcccag ttggtgaccc 841 tgaaggtggg gaacaagagc agagtcctgg agctgcagtt tgggatgaat gccacttcta 901 gcctgttttt cctgcaagga gttcagttga acatgactct tcctgatgcc atagagccca 961 cgttcagcac ctccaactat tccctgaaag ctcttcaggc cagtgtcggc aactcataca 1021 agtgcaacag tgaggagcac atctttgtca gcaaggcgct cgccctcaat gtcttcagcg 1081 tgcaagtcca ggctttcagg gtagaaagtg acaggtttgg gtctgtggaa gagtgtgtac 1141 aggacggtaa caacatgctg atccccattg ctgtgggcgg ggccctggca gggctggtcc 1201 tcatcgtcct catcgcctac ctcatcggca ggaagaggag tcacgcgggc tatcagacca 1261 tctcggaatt cggctccacc ggctccaccg gctccaccgg cgcggatcca ccggtcgcca 1321 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 1381 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 1441 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 1501 ccctcgtgac caccctgagc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga 1561 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 1621 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 1681 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 1741 acaagctgga gtacaacgcc atccacggca acgtctatat caccgccgac aagcagaaga 1801 acggcatcaa ggccaacttc ggcctcaact gcaacatcga ggacggcagc gtgcagctcg 1861 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 1921 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg 1981 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 2041 aaagcggccg cgactctaga tcataatcag ccataccaca ttt //