LOCUS Exported 2127 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Streptactin-tag. ACCESSION . VERSION . KEYWORDS StrepII-tag SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2127) AUTHORS Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T TITLE The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. JOURNAL J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29. PUBMED 24295448 REFERENCE 2 (bases 1 to 2127) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2127 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 46..69 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" misc_feature 79..99 /label=BioBrick suffix /note="universal suffix for all parts" terminator 100..157 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(181..198) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(352..940) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(432..451) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1123..1217 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1238..1897) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1812..1831 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 2059..2077 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2081..2124 /label=bacterial terminator /note="putative bacterial transcription terminator" ORIGIN 1 aattccgcgg ccgcttctag ataaggagga actactatgg ccggctggag ccacccgcag 61 ttcgaaaaaa ccggttaata ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt 121 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc 181 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 241 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 301 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag 361 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 421 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 481 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 541 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 601 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 661 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 721 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 781 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 841 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 901 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 961 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 1021 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 1081 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa 1141 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg 1201 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat 1261 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat 1321 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca 1381 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga 1441 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat 1501 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga 1561 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg 1621 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa 1681 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt 1741 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat 1801 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata 1861 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa 1921 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg 1981 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc 2041 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc 2101 ttagctttcg ctaaggatga tttctgg //