LOCUS Exported 2227 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Strong constitutive promoter. ACCESSION . VERSION . KEYWORDS PlepA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2227) AUTHORS Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T TITLE The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. JOURNAL J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29. PUBMED 24295448 REFERENCE 2 (bases 1 to 2227) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2227 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 179..199 /label=BioBrick suffix /note="universal suffix for all parts" terminator 200..257 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(281..298) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(452..1040) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(532..551) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1223..1317 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1338..1997) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1912..1931 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 2159..2177 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2181..2224 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2227..21 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aattcgcggc cgcttctaga gagtcaatgt atgaatggat acgggatatg aatcaataag 61 tacgtgaaag agaaaagcaa cccagatatg atagggaact tttctctttc ttgttttaca 121 ttgaatcttt acaatcctat tgatataatc taagctagtg tattttgcgt ttaatagtta 181 ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt 241 ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc 301 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc 481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 1021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 1081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 1141 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 1201 ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg 1261 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa 1321 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca 1381 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc 1441 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag 1501 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct 1561 gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa 1621 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc 1681 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta 1741 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc 1801 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc 1861 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac 1921 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca 1981 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat 2041 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc 2101 aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 2161 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga 2221 tttctgg //