LOCUS       Exported                2420 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Xylose-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    PxylA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      6..63
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(87..104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(258..846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(338..357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1017..1877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1640..1659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1878..1982)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2050..2068
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      2072..2115
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2118..2139
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    2405..5
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
       61 agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      121 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      181 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      241 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
      301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      421 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      661 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      841 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      961 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1021 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1081 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1141 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1201 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1261 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1321 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1381 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1441 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1501 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1561 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1621 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1681 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1741 tcgatataac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1801 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1861 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1921 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1981 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2041 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     2101 gctaaggatg atttctggaa ttcgcggccg cttctagaga aggccaaaaa actgctgcct
     2161 tcggatcagc gatatccact tcatccactc catttgttta atctttaaat taagtatcaa
     2221 catagtacat agcgaatctt ccctttatta tatctaatgt gttcataaaa aactaaaaaa
     2281 aatattgaaa atactgacga ggttatataa gatgaaaata agttagtttg tttaaacaac
     2341 aaactaatag gtgatgtact tactatatga aataaaatgc atctgggatc ccaagcttat
     2401 cgaatactag tagcggccgc
//
