LOCUS       Exported                 994 bp ds-DNA     linear   SYN 09-JUN-2021
DEFINITION  Expresses active site tyrosine mutated HA-tagged mouse DNA 
            topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone).
ACCESSION   .
VERSION     .
KEYWORDS    pYLL204
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Ban Y, Ho CW, Lin RK, Lyu YL, Liu LF
  TITLE     Activation of a novel ubiquitin-independent proteasome pathway when 
            RNA polymerase II encounters a protein roadblock.
  JOURNAL   Mol Cell Biol. 2013 Oct;33(20):4008-16. doi: 10.1128/MCB.00403-13. 
            Epub 2013 Aug 12.
  PUBMED    23938298
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        52..70
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     52..69
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             119..145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt gatttaggtg
       61 acactataga atacaagcta cttgttcttt ttgcaggatc ccatcgatag ccaccatgta
      121 cccatacgac gtcccagact acgctgccaa gtccagcctc gcgggctctg acggggcgct
      181 gacctgggtg aacaatgcta caaaaaagga agagttggag acagcaaaca aaaatgactc
      241 tactaagaag ttgtctgttg agagagtgta ccagaagaag acccaactgg agcacattct
      301 ccttcgtcct gatacataca ttggatcagt ggagccactg acacagctaa tgtgggtgta
      361 tgatgaagat gtagggatga actgcagaga ggttaccttt gttccaggct tatacaagat
      421 ctttgatgaa attttggtta atgctgctga caacaagcag agggacaaga acatgacctg
      481 tattaaggtt tctattgatc ctgaatctaa tattataagc atttggaata atgggaaagg
      541 cattccagta gtagaacaca aagtagagaa agtttatgtt cctgctttaa tttttggaca
      601 gcttttaaca tcgagtaatt atgacgatga tgagaaaaaa gttacaggag gccgtaatgg
      661 ttatggtgca aagctttgta atatttttag tacaaagttt acagtagaaa cggcttgcaa
      721 agaatacaaa catagtttta aacagacatg gatgaataat atgatgaaga cttctgaagc
      781 caaaattaaa cattttgatg gtgaagatta tacatgcata acattccagc cagatctatc
      841 caaatttaag atggaaaaac ttgacaagga cattgtggcc ctcatgacca gaagagtata
      901 tgatttagct gggtcctgta aaggagtcaa ggtcatgttt aatgggaaga agttgcctgt
      961 aaatggattt cgcagctatg tagaccttta tgtg
//
