LOCUS Exported 1071 bp ds-DNA linear SYN 12-JUN-2021 DEFINITION bacterial expression plasmid. ACCESSION . VERSION . KEYWORDS MTRA-c034 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1071) TITLE SGC_OxfordQ1Q2_2014 REFERENCE 2 (bases 1 to 1071) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1071 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 7..29 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 40..57 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 82..102 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" ORIGIN 1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct 61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgaaaac cctgcgggat 121 gagatcaatg ccattctgca gaagaggatt atggtgctgg atggagggat ggggaccatg 181 atccagcggg agaagctaaa cgaagaacac ttccgaggtc aggaatttaa agatcatgcc 241 aggccgctga aaggcaacaa tgacatttta agtataactc agcctgatgt catttaccaa 301 atccataagg aatacttgct ggctggggca gatatcattg aaacaaatac ttttagcagc 361 actagtattg cccaagctga ctatggcctt gaacacttgg cctaccggat gaacatgtgc 421 tctgcaggag tggccagaaa agctgccgag gaggtaactc tccagacagg aattaagagg 481 tttgtggcag gggctctggg tccgactaat aagacactct ctgtgtcccc atctgtggaa 541 aggccggatt ataggaacat cacatttgat gagcttgttg aagcatacca agagcaggcc 601 aaaggacttc tggatggcgg ggttgatatc ttactcattg aaactatttt tgatactgcc 661 aatgccaagg cagccttgtt tgcactccaa aatctttttg aggagaaata tgctccccgg 721 cctatcttta tttcagggac gatcgttgat aaaagtgggc ggactctttc cggacagaca 781 ggagagggat ttgtcatcag cgtgtctcat ggagaaccac tctgcattgg attaaattgt 841 gctttgggtg cagctgaaat gagacctttt attgaaataa ttggaaaatg tacaacagcc 901 tatgtcctct gttatcccaa tgcaggtctt cccaacacct ttggtgacta tgatgaaacg 961 ccttctatga tggccaagca cctaaaggat tttgctatgg atggcttggt caatatagtt 1021 ggaggatgct gtgggtcaac accagatcat atcagggaaa ttgctgaagc t //