LOCUS       Exported                1930 bp ds-DNA     linear   SYN 15-JUN-2021
DEFINITION  Localization: Actin, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    mPlum-Actin-C-18
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1930)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             27..704
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     428..447
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     622..645
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atccgctagc gctaccggtg gctaccatgg tgagcaaggg cgaggaggtc atcaaggagt
       61 tcatgcgctt caaggagcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      121 gcgagggcga gggccgcccc tacgagggca cccagaccgc caggctgaag gtgaccaagg
      181 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gatcatgtac ggctccaagg
      241 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      301 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      361 cctccctgca ggacggcgag ttcatctaca aggtgaaggt gcgcggcacc aacttcccct
      421 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      481 accccgagga cggcgccctg aagggcgaga tgaagatgag gctgaggctg aaggacggcg
      541 gccactacga cgccgaggtc aagaccacct acatggccaa gaagcccgtg cagctgcccg
      601 gcgcctacaa gaccgacatc aagctggaca tcacctccca caacgaggac tacaccatcg
      661 tggaacagta cgagcgcgcc gagggccgcc actccaccgg cgcctccgga ctcagatctg
      721 gcagcggtgg aggcagcgca tccggcggaa gcggaagcga tgatgatatc gccgcgctcg
      781 tcgtcgacaa cggctccggc atgtgcaagg ccggcttcgc gggcgacgat gccccccggg
      841 ccgtcttccc ctccatcgtg gggcgcccca ggcaccaggg cgtgatggtg ggcatgggtc
      901 agaaggattc ctatgtgggc gacgaggccc agagcaagag aggcatcctc accctgaagt
      961 accccatcga gcacggcatc gtcaccaact gggacgacat ggagaaaatc tggcaccaca
     1021 ccttctacaa tgagctgcgt gtggctcccg aggagcaccc cgtgctgctg accgaggccc
     1081 ccctgaaccc caaggccaac cgcgagaaga tgacccagat catgtttgag accttcaaca
     1141 ccccagccat gtacgttgct atccaggctg tgctatccct gtacgcctct ggccgtacca
     1201 ctggcatcgt gatggactcc ggtgacgggg tcacccacac tgtgcccatc tacgaggggt
     1261 atgccctccc ccatgccatc ctgcgtctgg acctggctgg ccgggacctg actgactacc
     1321 tcatgaagat cctcaccgag cgcggctaca gcttcaccac cacggccgag cgggaaatcg
     1381 tgcgtgacat taaggagaag ctgtgctacg tcgccctgga cttcgagcaa gagatggcca
     1441 cggctgcttc cagctcctcc ctggagaaga gctacgagct gcctgacggc caggtcatca
     1501 ccattggcaa tgagcggttc cgctgccctg aggcactctt ccagccttcc ttcctgggca
     1561 tggagtcctg tggcatccac gaaactacct tcaactccat catgaagtgt gacgtggaca
     1621 tccgcaaaga cctgtacgcc aacacagtgc tgtctggcgg caccaccatg taccctggca
     1681 ttgccgacag gatgcagaag gagatcactg ccctggcacc cagcacaatg aagatcaaga
     1741 tcattgctcc tcctgagcgc aagtactccg tgtggatcgg cggctccatc ctggcctcgc
     1801 tgtccacctt ccagcagatg tggatcagca agcaggagta tgacgagtcc ggcccctcca
     1861 tcgtccaccg caaatgcttc tagggatcca ccggatctag ataactgatc ataatcagcc
     1921 ataccacatt
//
