LOCUS       Exported                3521 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 590, 
            Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    mPlum-CAF1-C-10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3521)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             34..711
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     435..454
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     629..652
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    2530..2541
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 acgtcagatc cgctagcgct accggtggct accatggtga gcaagggcga ggaggtcatc
       61 aaggagttca tgcgcttcaa ggagcacatg gagggctccg tgaacggcca cgagttcgag
      121 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccag gctgaaggtg
      181 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagat catgtacggc
      241 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      301 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      361 caggactcct ccctgcagga cggcgagttc atctacaagg tgaaggtgcg cggcaccaac
      421 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      481 cggatgtacc ccgaggacgg cgccctgaag ggcgagatga agatgaggct gaggctgaag
      541 gacggcggcc actacgacgc cgaggtcaag accacctaca tggccaagaa gcccgtgcag
      601 ctgcccggcg cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac
      661 accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgc ctccggactc
      721 agatctggca gcggtggagg cttggaggag ccggaggccg cgacgcggac agccgcggcc
      781 gtggattgca aagacagacc cgggttccca gtgaagaggt taatacaagc ccgcttgccc
      841 ttcaaacgcc tgaaccttgt tcccaaggag aaagttgagg aagacacatc accaaaagca
      901 gctgtggaaa gcaaagtccc ggatctacaa ttgtctttgg gcacttttga aagccagtgt
      961 cacacaggct ctcacgtagg cttgagtaca aagttggtcg gtggccaggg ccccattgat
     1021 agcttcttga gggcaacaat caagccagta ccgagtgtgg tcattatcga cctgacggag
     1081 aactgcagtg acattccaga cagccctgag ggccacagtg aactaagtcc tgacactgct
     1141 ggtgtggtca ccacagtgga aggagctgcc aagcagcagg agcactctgc agcagagctg
     1201 tgtctcctag agaccccttc agacatcact tgtcacatgg aagaggagcc tgggagtcca
     1261 ggagacccaa agaggacggg tgactgtcag gctggttcac tacagagctg tccggagctg
     1321 accccaggtt ctaggacatg ccccaccaag gagttatcaa gctggagcaa ggcaggggat
     1381 ctcctattta tagagaaagt gcctgtggtg gtgctagagg acatcctggc cactaagccc
     1441 tccattgcct ctcttcccat gatgtccctg gataggagtg tcacttcaga gagtgaaata
     1501 ctggagtcct gccccgagga tgactccata ctgagccact cctccaccaa ctcctcttct
     1561 ccaaccagct ctcctgaggg accatccaca cccccagaac atcggggtgg gagaagctcc
     1621 ccctccaccc cagcctgcag agttgccaag aactttgtca aaggctccac agagaagggc
     1681 aggagcaaac tgcacagaga tagagaacag cagagggaag agaaggaaaa gctgagggaa
     1741 gagatacgac gagccaagga ggaggcacgg aagaagaagg aggaggagaa ggagctgaag
     1801 gaaaaggagc ggagggaaaa gcgggagaaa gatgagaagg agaaggcgga gaagcagcgg
     1861 ctaaaggagg aaaaacgcaa ggagcggcag gaagctctgg aggctaaact ggaggagaag
     1921 aggaagaagg aggaagagaa gcggttacgg gaggaagaga agcggttacg ggaagaagag
     1981 aagcgcatta aggcagaaaa ggcagagatc actcgattct tccagaaacc gaagactccg
     2041 caggctccca agaccctggc cggttcctgt ggtaagtttg ccccctttga aatcaaagaa
     2101 cacatggtgc tggctccacg gtgccgagct gccttggatc aagacctgtg cgatcagctg
     2161 gaccagctcc tgcagcagca gagtgtggca agcaccttcc tcagtgacct aaaaagccgg
     2221 ctgcccttga gatcggggcc cacccgggtc tgtggccatg acacagacat catgaacagg
     2281 gatgtggtca ttgtggagag cagcaaggtg gacggtgtgt ctgaaaggaa gaagtttggc
     2341 cgcatgaagc ttctgcagtt ctcagagaac caccggccag catactgggg gacgtggaac
     2401 aagaagacag ccatcatccg cccccggaac ccctgggccc aggacaaaga ccttcttgac
     2461 tatgaggtag acagtgacga tgagtgggaa gaggaggaac ctggggagtc tttgtcccac
     2521 agtgaggggg atgaggatga cgatgtgggg gaagatgagg atgaggacga tggcttcttt
     2581 gtgccccatg ggtacttatc tgaagatgaa ggcgtgactg aggagtgtgc agaccctgag
     2641 aaccacaaag tacaccagaa gctgaaggcc aaggagtggg atgagctcct agccaagggc
     2701 aagcgctttc gtgtgctgca gcctgtgcac gtgggatgtg tatgggcatc agaggcagcc
     2761 aactgcacga gttccgactt gaagctgctg cagcagttca ctgcatgcct gctggatgtg
     2821 gcatcccctg atgagccaga acccggggcc tccaggaggg aaaagaggga ccagcatatc
     2881 ctggcccagt tgcttccgct gctacatggc aatgtgaatg ggagcaaggt gatcatccat
     2941 gaattccagg agcaatgccg caggggcctg ctgacccttc caagtcccac cccacacctg
     3001 cagatgccca acttggagga tgctgttgct gtgccatcca aggccaggct gaagcgcctt
     3061 atctctgaga actctgccta tgagaagcgg cccaacttcc gtatgtgctg gtatgtgcac
     3121 ccagaggtgc tcaagagctt tggccaggag tgcctgccag tgccctgcca gtggacctac
     3181 atcaccacca tgccctcagc acccagggaa gacagtggca gtgcttctac tgaggggcct
     3241 ggccagagca cacctatgct actcaagcgg aagccagcgg ccaccatgtg catcacgcag
     3301 tttatgaaga agcggcgcta tgatggacag gttgggtctg gggacatgga tggcttccag
     3361 gcagacacag aagaggatga agaggatgac acagactgta tgatcataga tgtgccagat
     3421 gttgggagtg acgtgtcaga agctcccata cctgctccca cactttgcaa gtgaggatcc
     3481 accggatcta gataactgat cataatcagc cataccacat t
//
