LOCUS       Exported                1797 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Localization: Lysosomes, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    mPlum-CathespinB-6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1797)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1078..1758
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     1479..1498
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1673..1696
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccagg
       61 atgtggtggt ccttgatccc tctctcttgc ctgctggcac tgaccagtgc ccatgacaag
      121 ccttcctttc acccactgtc ggacgacatg attaactata tcaacaaaca gaatacaaca
      181 tggcaggctg gacgcaactt ctacaatgtt gacataagct atctgaagaa gctgtgtgga
      241 actgtcctgg gtggacccaa gctgccggaa agggttgggt tcagcgagga cataaatcta
      301 cctgaatcct ttgatgcacg ggaacagtgg tccaattgcc cgaccatcgc acagatcaga
      361 gaccaggggt cctgtggctc ttgttgggca tttggggcag tggaagccat gtctgaccga
      421 atctgcattc acaccaatgg ccgagtcaat gtggaggtgt ctgctgagga cctgcttacc
      481 tgctgtggta tccagtgtgg ggatggctgt aatggtggct atccctctgg agcatggaac
      541 ttctggacta gaaaaggcct ggtttctggt ggagtataca attctcatat aggctgctta
      601 ccctacacca tccctccctg tgaacaccat gtcaatggct cccgtccccc atgcactgga
      661 gaaggagata ctcccaagtg caacaagatg tgtgaggctg gctactccac atcctacaag
      721 gaagataagc actatgggta cacttcctac agtgtgtctg acagcgagaa ggagatcatg
      781 gcggaaatct acaaaaatgg cccagtggag ggtgctttta ctgtgttttc tgacttcttg
      841 acttacaaat caggcgtata caagcatgaa gccggtgatg tgatgggagg ccatgccatc
      901 cgcattctgg gctggggaat agagaatgga gtaccctact ggctggtagc aaactcctgg
      961 aacgttgact ggggtgataa tggtttcttt aaaatcctca gaggagagaa ccactgtgga
     1021 attgaatcag aaatcgtggc tggaatccca cgcactcagg atccaccggt ggctaccatg
     1081 gtgagcaagg gcgaggaggt catcaaggag ttcatgcgct tcaaggagca catggagggc
     1141 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1201 acccagaccg ccaggctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1261 ctgtcccctc agatcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1321 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1381 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1441 aaggtgaagg tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     1501 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     1561 atgaagatga ggctgaggct gaaggacggc ggccactacg acgccgaggt caagaccacc
     1621 tacatggcca agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac
     1681 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc
     1741 cactccaccg gcgcctaagc ggccgcgact ctagatcata atcagccata ccacatt
//
