LOCUS       Exported                 800 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    mPlum-ER-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 800)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             73..750
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     474..493
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     668..691
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agatccgcta gcatgctgct atccgtgccg ttgctgctcg gcctcctcgg cctggccgtc
       61 gccgaccggt cgatggtgag caagggcgag gaggtcatca aggagttcat gcgcttcaag
      121 gagcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      181 cgcccctacg agggcaccca gaccgccagg ctgaaggtga ccaagggtgg ccccctgccc
      241 ttcgcctggg acatcctgtc ccctcagatc atgtacggct ccaaggccta cgtgaagcac
      301 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      361 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      421 ggcgagttca tctacaaggt gaaggtgcgc ggcaccaact tcccctccga cggccccgta
      481 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      541 gccctgaagg gcgagatgaa gatgaggctg aggctgaagg acggcggcca ctacgacgcc
      601 gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc
      661 gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
      721 cgcgccgagg gccgccactc caccggcgcc aaggacgagc tgtaagaatt ctgcagtcga
      781 cggtaccgcg ggcccgggat
//
