LOCUS       Exported                 886 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    mPlum-ER-5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 886)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             91..768
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     492..511
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     686..709
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgctg ctatccgtgc cgttgctgct cggcctcctc
       61 ggcctggccg tcgccgcacc ggtggctacc atggtgagca agggcgagga ggtcatcaag
      121 gagttcatgc gcttcaagga gcacatggag ggctccgtga acggccacga gttcgagatc
      181 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaggct gaaggtgacc
      241 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagatcat gtacggctcc
      301 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      361 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      421 gactcctccc tgcaggacgg cgagttcatc tacaaggtga aggtgcgcgg caccaacttc
      481 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      541 atgtaccccg aggacggcgc cctgaagggc gagatgaaga tgaggctgag gctgaaggac
      601 ggcggccact acgacgccga ggtcaagacc acctacatgg ccaagaagcc cgtgcagctg
      661 cccggcgcct acaagaccga catcaagctg gacatcacct cccacaacga ggactacacc
      721 atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcgcctc cggaaaggac
      781 gagctgtaag aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata
      841 actgatcata atcagccata ccacatttgt agaggtttac ttgctt
//
