LOCUS       Exported                1104 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    mPlum-MannII-N-10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1104)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             385..1065
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     786..805
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     980..1003
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agatccgcta gcgccaccat gaagttaagt cgccagttca ccgtgtttgg cagcgcgatc
       61 ttctgcgtcg taatcttctc actctacctg atgctggaca ggggtcactt ggactaccct
      121 cggggcccgc gccaggaggg ctcctttccg cagggccagc tttcaatatt gcaagaaaag
      181 attgaccatt tggagcgttt gctcgctgag aacaacgaga tcatctcaaa tatcagagac
      241 tcagtcatca acctgagcga gtctgtggag gacggcccgc gggggtcacc aggcaacgcc
      301 agccaaggct ccatccacct ccactcgcca cagttggccc tgcaggctga ccccagctcc
      361 ggatcggatc caccggtggc taccatggtg agcaagggcg aggaggtcat caaggagttc
      421 atgcgcttca aggagcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      481 gagggcgagg gccgccccta cgagggcacc cagaccgcca ggctgaaggt gaccaagggt
      541 ggccccctgc ccttcgcctg ggacatcctg tcccctcaga tcatgtacgg ctccaaggcc
      601 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      661 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      721 tccctgcagg acggcgagtt catctacaag gtgaaggtgc gcggcaccaa cttcccctcc
      781 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
      841 cccgaggacg gcgccctgaa gggcgagatg aagatgaggc tgaggctgaa ggacggcggc
      901 cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca gctgcccggc
      961 gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta caccatcgtg
     1021 gaacagtacg agcgcgccga gggccgccac tccaccggcg cctaagcggc cgcgactcta
     1081 gatcataatc agccatacca catt
//
