LOCUS       Exported                 994 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  Localization: Nucleus, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    mPlum-Nucleus-7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        23..74
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     25..45
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             119..796
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     520..539
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     714..737
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             821..841
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             845..865
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             869..889
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
       61 atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg tggctaccat
      121 ggtgagcaag ggcgaggagg tcatcaagga gttcatgcgc ttcaaggagc acatggaggg
      181 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      241 cacccagacc gccaggctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      301 cctgtcccct cagatcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
      361 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
      421 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
      481 caaggtgaag gtgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
      541 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
      601 gatgaagatg aggctgaggc tgaaggacgg cggccactac gacgccgagg tcaagaccac
      661 ctacatggcc aagaagcccg tgcagctgcc cggcgcctac aagaccgaca tcaagctgga
      721 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgcg ccgagggccg
      781 ccactccacc ggcgcctccg gactcagatc tcgagctgat ccaaaaaaga agagaaaggt
      841 agatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaaggtag gatccaccgg
      901 atctagataa ctgatcataa tcagccatac cacattctga tcataatcag ccataccaca
      961 tttgtagagg ttttacttgc tttaaaaaac ctcc
//
