LOCUS       Exported                9014 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    EF.mHES1.CMV.GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI
  TITLE     HES1 inhibits cycling of hematopoietic progenitor cells via DNA 
            binding.
  JOURNAL   Stem Cells. 2006 Apr . 24(4):876-88.
  PUBMED    16513761
REFERENCE   2  (bases 1 to 9014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2399..2656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2700..2825
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3318..3551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3735..3779
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4169..5347
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4400..5338
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5295..5315
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5364..5382
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        6343..6646
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6647..6850
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6801..6821
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      6932..6941
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6938..7657
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             6938..7654
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6983..7004)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7244..7263)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7591..7612
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             7755..7988
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8060..8181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8097..8116)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8151..8170
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8200..8335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8262..8281
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        complement(8368..8386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8396..8413)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8396..8412)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8405..8427)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8557..9012
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8644..8663)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8854..8875
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgga
     2401 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     2461 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2521 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2581 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2641 tggaaaatct ctagcagtgg cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc
     2701 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     2761 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     2821 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     2881 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     2941 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     3001 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     3061 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     3121 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     3181 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     3241 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     3301 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     3361 atgggcgcag cctcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     3421 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     3481 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     3541 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     3601 tggaatgcta gttggagtaa taaatctctg gaacagattg gaatcacacg acctggatgg
     3661 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     3721 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     3781 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     3841 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     3901 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     3961 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     4021 acggatctcg acggtatcga tcacgagact agcctcgaga agcttgatga cggtatcgat
     4081 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
     4141 tctaggtctt gaaaggagtg cctcgtgagg ctccggtgcc cgtcagtggg cagagcgcac
     4201 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     4261 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     4321 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     4381 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     4441 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     4501 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     4561 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     4621 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     4681 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     4741 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     4801 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     4861 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     4921 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     4981 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     5041 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     5101 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     5161 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     5221 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     5281 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     5341 gtcgtgagga attagcttgg tactaatacg actcactata gggagaccca agctggctag
     5401 ttaagcttga taattcatgc cagctgatat aatggagaaa aattcctcct ccccggtggc
     5461 tgctacccca gccagtgtca acacgacacc ggacaaacca aagacggcct ctgagcacag
     5521 aaagtcatca aagcctatca tggagaagag gcgaagggca agaataaatg aaagtctaag
     5581 ccaactgaaa acactgattt tggatgcact taagaaagat agctcccggc attccaagct
     5641 agagaaggca gacattctgg aaatgactgt gaagcacctc cggaacctgc agcgggcgca
     5701 gatgaccgcc gcgctcagca cagacccgag cgtgttgggg aaataccgcg ccggcttcag
     5761 cgagtgcatg aacgaggtga cccgcttcct gtccacgtgt gagggcgtta acaccgaggt
     5821 gcgcactcgg ctgctgggcc acctggccaa ctgcatgacc cagatcaacg ccatgaccta
     5881 ccccgggcag gcgcaccccg ccttgcaggc gccgccgccg ccgcccccgt caggacctgc
     5941 cggtccccag cacgcgccat tcgcgccgcc gccgccgccg cttgtgccca tccccggggg
     6001 cgcggcgccc cctcccggca gcgcaccctg caagttgggc agccaggctg gagaggctgc
     6061 caaggttttt ggcggcttcc aagtggtgcc ggctcctgac ggccaatttg cctttctcat
     6121 ccccaacggg gccttcgctc acagcggccc ggtcatcccg gtctacacca gcaacagtgg
     6181 gacctcggtg ggtcctaacg cagtgtcacc ttccagtggc tcctcgctca cttcggactc
     6241 catgtggaga ccgtggcgga actgagcggc catcgaattc ctgcagccct aatagtaatc
     6301 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     6361 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     6421 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     6481 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     6541 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     6601 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     6661 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     6721 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     6781 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     6841 aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggactc agatctcgag
     6901 ctcaagcttc gaattggggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct
     6961 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     7021 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     7081 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     7141 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     7201 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     7261 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     7321 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     7381 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     7441 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     7501 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     7561 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     7621 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgtcg acgggcccgc
     7681 ggaattccgt ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7741 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     7801 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7861 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7921 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7981 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     8041 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     8101 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8161 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     8221 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8281 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8341 ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     8401 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     8461 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     8521 cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc ggcgcattaa
     8581 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     8641 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     8701 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     8761 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     8821 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     8881 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     8941 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     9001 cgtttacaat ttcc
//
