LOCUS Exported 12262 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS EF.mHES1.Ubc.GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12262) AUTHORS Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI TITLE HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding. JOURNAL Stem Cells. 2006 Apr . 24(4):876-88. PUBMED 16513761 REFERENCE 2 (bases 1 to 12262) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12262 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" primer_bind 2624..2640 /label=KS primer /note="common sequencing primer, one of multiple similar variants" promoter 2770..3948 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 3001..3939 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3896..3916 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" primer_bind 3965..3984 /label=T7 /note="T7 promoter, forward primer" promoter 3965..3983 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 5979..5998 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" regulatory 6188..6197 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6194..6913 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6194..6910 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6239..6260) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6500..6519) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6847..6868 /label=EGFP-C /note="EGFP, forward primer" misc_feature 6954..7542 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7007..7027) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7425..7436) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(7545..7561) /label=KS primer /note="common sequencing primer, one of multiple similar variants" LTR 8067..8247 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(8273..8290) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8279..8503 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8549..8977 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8636..8655) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8846..8867 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8986..9006) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8991..9320 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9171..9306 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9233..9252 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 9368..9415 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9434..9808 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 9938..10059 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9975..9994) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10029..10048 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(10108..10124) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10108..10124) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10121..10143) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10132..10148 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10156..10186) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10201..10222 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10339..10356) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10510..11098) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10590..10609) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11269..12129) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11892..11911 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12130..12234) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta agtttaaacg ggccctctag actcgaggtc gacggtatcg 2641 atcacgagac tagcctcgag aagcttgatg acggtatcga taagctttgc aaagatggat 2701 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt 2761 gcctcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 2821 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac 2881 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 2941 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 3001 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg 3061 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga 3121 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt 3181 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt 3241 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt 3301 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt 3361 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg 3421 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct 3481 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg 3541 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca 3601 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg 3661 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg 3721 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt 3781 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac 3841 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag 3901 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattagcttg 3961 gtactaatac gactcactat agggagaccc aagctggcta gttaagcttg ataattcatg 4021 ccagctgata taatggagaa aaattcctcc tccccggtgg ctgctacccc agccagtgtc 4081 aacacgacac cggacaaacc aaagacggcc tctgagcaca gaaagtcatc aaagcctatc 4141 atggagaaga ggcgaagggc aagaataaat gaaagtctaa gccaactgaa aacactgatt 4201 ttggatgcac ttaagaaaga tagctcccgg cattccaagc tagagaaggc agacattctg 4261 gaaatgactg tgaagcacct ccggaacctg cagcgggcgc agatgaccgc cgcgctcagc 4321 acagacccga gcgtgttggg gaaataccgc gccggcttca gcgagtgcat gaacgaggtg 4381 acccgcttcc tgtccacgtg tgagggcgtt aacaccgagg tgcgcactcg gctgctgggc 4441 cacctggcca actgcatgac ccagatcaac gccatgacct accccgggca ggcgcacccc 4501 gccttgcagg cgccgccgcc gccgcccccg tcaggacctg ccggtcccca gcacgcgcca 4561 ttcgcgccgc cgccgccgcc gcttgtgccc atccccgggg gcgcggcgcc ccctcccggc 4621 agcgcaccct gcaagttggg cagccaggct ggagaggctg ccaaggtttt tggcggcttc 4681 caagtggtgc cggctcctga cggccaattt gcctttctca tccccaacgg ggccttcgct 4741 cacagcggcc cggtcatccc ggtctacacc agcaacagtg ggacctcggt gggtcctaac 4801 gcagtgtcac cttccagtgg ctcctcgctc acttcggact ccatgtggag accgtggcgg 4861 aactgagcgg ccatcgaatt cccaccacac tggactagtg gatccggggg taccgcgtcg 4921 aggggtgcag cggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac 4981 ggcgagcgct gccacgtcag acgaagggcg caggagcgtt cctgatcctt ccgcccggac 5041 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa 5101 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg 5161 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg 5221 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc 5281 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagttgcgg 5341 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt 5401 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt 5461 tggggggagc gcacaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtaagg 5521 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc 5581 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc 5641 tggggaccct gacgtgaagt ttgtcactga ctggagaact cgggtttgtc gtctggttgc 5701 gggggcggca gttatgcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc 5761 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc 5821 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc 5881 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt 5941 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat 6001 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc 6061 atttgggtca atatgtaatt ttcagtgtta gactagtaaa gcttctgcag gtcgactcta 6121 gaaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacagg atccccgggt 6181 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 6241 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 6301 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 6361 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 6421 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 6481 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 6541 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 6601 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 6661 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 6721 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 6781 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 6841 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 6901 gctgtacaag taaagcggcc gcgactctag aattcgatat caagcttatc gataatcaac 6961 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 7021 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 7081 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 7141 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 7201 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 7261 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 7321 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg 7381 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 7441 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 7501 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc 7561 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt 7621 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac 7681 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 7741 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct 7801 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat 7861 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag 7921 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg 7981 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg 8041 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg 8101 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 8161 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 8221 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact 8281 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 8341 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 8401 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 8461 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 8521 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 8581 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 8641 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 8701 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 8761 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 8821 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 8881 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 8941 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 9001 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 9061 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 9121 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 9181 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 9241 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 9301 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 9361 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt 9421 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt 9481 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga 9541 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca 9601 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc 9661 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga 9721 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt 9781 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc 9841 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 9901 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 9961 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 10021 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 10081 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 10141 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 10201 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 10261 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 10321 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 10381 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 10441 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 10501 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 10561 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 10621 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 10681 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 10741 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 10801 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 10861 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 10921 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 10981 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 11041 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 11101 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 11161 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 11221 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 11281 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 11341 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 11401 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 11461 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 11521 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 11581 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 11641 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 11701 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 11761 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 11821 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 11881 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 11941 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 12001 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 12061 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 12121 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 12181 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 12241 tccccgaaaa gtgccacctg ac //