LOCUS       Exported                1888 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Localization: Focal Adhesions, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    mIFP12-LASP1-C-10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             33..992
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     regulatory      1243..1252
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatgtcggt accgctgact acctcagcat
       61 tcggccacgc gtttctggct aactgtgaac gcgagcagat ccacctggcg ggctccattc
      121 agccgcacgg tatcctgctg gctgtgaaag agccggacaa cgtggtgatc caggcttcta
      181 ttaacgctgc ggagttcctg aacaccaact ctgttgttgg ccgtccgctg cgtgacctgg
      241 gcggcgatct gcctttgcag atcctgccgc acctgaacgg cccgctgcac ctggctccga
      301 tgaccctgcg ttgtaccgtg ggttctccgc cgcgtcgtgt ggactgtacc attcatcgtc
      361 cgtctaacgg cggcctgatc gtagaactgg aaccagcaac caagaccact aacattgcgc
      421 cggctctgga cggtgcgttt catcgtatca cttcttcatc ctccctgatg ggcctgtgtg
      481 acgaaaccgc gactattatc cgtgagatta ctggctacga ccgtgtgatg gtagtacgtt
      541 tcgatgaaga gggtaatggc gaaattctgt ccgaacgtcg tcgtgcggac ctggaagcgt
      601 tcctgggtaa ccgctacccg gcgtctacta ttccgcagat cgctcgtcgc ctgtacgaac
      661 ataaccgtgt tcgcctgctg gtagatgtga actatactcc ggttccgcta cagccgcgca
      721 tcagcccgct gaacggtcgt gatctggata tgtccctgtc ttgcctgcgc tctatgtccc
      781 cgatccacca gaaatacatg caggacatgg gcgttggcgc gaccctggtt tgctctctga
      841 tggtgtctgg tcgtctgtgg ggtctgatcg cttgccacca ctacgaaccg cgcttcgttc
      901 cgttccacat tcgcgctgct ggcgaagcgc tggcggaaac ttgtgcgatc cgcatcgcga
      961 cgctggagag ctttgcacag tctcagtcca aaggtggcgg aagcggcggc ggaagcggtg
     1021 gcggaagttc cggactcaga tctagcggtg gcagtggaaa ccccaactgc gcccggtgcg
     1081 gcaagatcgt gtatcccacg gagaaggtga actgtctgga taagttctgg cataaagcat
     1141 gcttccattg cgagacctgc aagatgacac tgaacatgaa gaactacaag ggctacgaga
     1201 agaagcccta ctgcaacgca cactacccca agcagtcctt caccatggtg gcggacaccc
     1261 cggaaaacct tcgcctcaag caacagagtg agctccagag tcaggtgcgc tacaaggagg
     1321 agtttgagaa gaacaagggc aaaggtttca gcgtagtggc agacacgccc gagctccaga
     1381 gaatcaagaa gacccaggac cagatcagta acataaaata ccatgaggag tttgagaaga
     1441 gccgcatggg ccctagcggg ggcgagggca tggagccaga gcgtcgggat tcacaggacg
     1501 gcagcagcta ccggcggccc ctggagcagc agcagcctca ccacatcccg accagtgccc
     1561 cggtttacca gcagccccag cagcagccgg tggcccagtc ctatggtggc tacaaggagc
     1621 ctgcagcccc agtctccata cagcgcagcg ccccaggtgg tggcgggaag cggtaccgcg
     1681 tggtgtatga ctacagcgcc gccgacgagg acgaggtctc cttccaggac ggggacacca
     1741 tcgtcaacgt gcagcagatc gacgacggct ggatgtacgg gacggtggag cgcaccggcg
     1801 acacggggat gctgccggcc aactacgtgg aggccatctg aggatccacc ggatctagat
     1861 aactgatcat aatcagccat accacatt
//
