LOCUS Exported 2236 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION Localization: Intermediate Filaments, Excitation: 488, Emission: 507. ACCESSION . VERSION . KEYWORDS EGFP-Vimentin-7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2236) TITLE Davidson Green fluorescent proteins REFERENCE 2 (bases 1 to 2236) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2236 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 1471..1480 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1477..2196 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 1477..2193 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1522..1543) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1783..1802) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2130..2151 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattcccatg 61 tccaccaggt ccgtgtcctc gtcctcctac cgcaggatgt tcggcggccc gggcaccgcg 121 agccggccga gctccagccg gagctacgtg actacgtcca cccgcaccta cagcctgggc 181 agcgcgctgc gccccagcac cagccgcagc ctctacgcct cgtccccggg cggcgtgtat 241 gccacgcgct cctctgccgt gcgcctgcgg agcagcgtgc ccggggtgcg gctcctgcag 301 gactcggtgg acttctcgct ggccgacgcc atcaacaccg agttcaagaa cacccgcacc 361 aacgagaagg tggagctgca ggagctgaat gaccgcttcg ccaactacat cgacaaggtg 421 cgcttcctgg agcagcagaa taagatcctg ctggccgagc tcgagcagct caagggccaa 481 ggcaagtcgc gcctggggga cctctacgag gaggagatgc gggagctgcg ccggcaggtg 541 gaccagctaa ccaacgacaa agcccgcgtc gaggtggagc gcgacaacct ggccgaggac 601 atcatgcgcc tccgggagaa attgcaggag gagatgcttc agagagagga agccgaaaac 661 accctgcaat ctttcagaca ggatgttgac aatgcgtctc tggcacgtct tgaccttgaa 721 cgcaaagtgg aatctttgca agaagagatt gcctttttga agaaactcca cgaagaggaa 781 atccaggagc tgcaggctca gattcaggaa cagcatgtcc aaatcgatgt ggatgtttcc 841 aagcctgacc tcacggctgc cctgcgtgac gtacgtcagc aatatgaaag tgtggctgcc 901 aagaacctgc aggaggcaga agaatggtac aaatccaagt ttgctgacct ctctgaggct 961 gccaaccgga acaatgacgc cctgcgccag gcaaagcagg agtccactga gtaccggaga 1021 caggtgcagt ccctcacctg tgaagtggat gcccttaaag gaaccaatga gtccctggaa 1081 cgccagatgc gtgaaatgga agagaacttt gccgttgaag ctgctaacta ccaagacact 1141 attggccgcc tgcaggatga gattcagaat atgaaggagg aaatggctcg tcaccttcgt 1201 gaataccaag acctgctcaa tgttaagatg gcccttgaca ttgagattgc cacctacagg 1261 aagctgctgg aaggcgagga gagcaggatt tctctgcctc ttccaaactt ttcctccctg 1321 aacctgaggg aaactaatct ggattcactc cctctggttg atacccactc aaaaaggaca 1381 cttctgatta agacggttga aactagagat ggacaggtta tcaacgaaac ttctcagcat 1441 cacgatgacc ttgaagggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg 1501 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 1561 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1621 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 1681 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 1741 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 1801 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 1861 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 1921 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 1981 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 2041 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 2101 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 2161 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa 2221 tcagccatac cacatt //