LOCUS       Exported                5538 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSE100
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein 
            M, Ehrt S, Schnappinger D
  TITLE     Silencing Mycobacterium smegmatis by using tetracycline repressors.
  JOURNAL   J Bacteriol. 2007 Jul . 189(13):4614-23.
  PUBMED    17483222
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      14..41
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    242..259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(481..497)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(482..498)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     rep_origin      4703..5291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5192..5211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5445..5462
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgattagcta agcagaaggc catcctgacg gatggccttt ttgcgtttaa tactgtttaa
       61 acctctagaa atattggatc gtcggcaccg tcacggccgt gggaggcggc acgatccgcg
      121 acgtgatgat cggccgcatc cccacggtgc tgcgcagtga gctctacgcc atcccggcgt
      181 tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg agcgggagaa
      241 ctccctatca gtgatagagt ttgtcctccc tatcagtgat agataggctc tgggagtacc
      301 cgtgtgtacg accagcacgg catacatcat ttcgacgccg agagattcgc cgcccgaaat
      361 gagcacgatc cgcatgctta attaacagct gatttaaatg gatcccccgg gctgcaggaa
      421 ttcgatatca agctttagct aattaattgg ggaccctaga ggtccccttt tttattttat
      481 cgataccgtc gacctcgagg gggggcccgc cggggatcat cgagccgaga acgttatcga
      541 agttggtcat gtgtaatccc ctcgtttgaa ctttggatta agcgtagata cacccttgga
      601 caagccagtt ggattcggag acaagcaaat tcagccttaa aaagggcgag gcctgcggtg
      661 gtggaacacc gcagggcctc taaccgctcg acgcgctgca ccaaccagcc cgcgaacggc
      721 tggcagccag cgtaaggcgc ggctcatcgg gcggcgttcg ccacgatgtc ctgcacttcg
      781 agccaagcct cgaacacctg ctggtgtgca cgactcaccc ggttgttgac accgcgcgcg
      841 gccgtgcggg ctcggtgggg cggctgtgtc gcccttgcca gcgtgagtag cgcgtacctc
      901 acctcgccca acaggtcgca cacagccgat tcgtacgcca taaagccagg tgagcccacc
      961 agctccgtaa gttcgggcgc tgtgtggctc gtacccgcgc attcaggcgg cagggggtct
     1021 aacgggtcta aggcggcgtg tacggccgcc acagcggctc tcagcggccc ggaaacgtcc
     1081 tcgaaacgac gcatgtgttc ctcctggttg gtacaggtgg ttgggggtgc tcggctgtcg
     1141 ctggtgttcc accaccaggg ctcgacggga gagcggggga gtgtgcagtt gtggggtggc
     1201 ccctcagcga aatatctgac ttggagctcg tgtcggacca tacaccggtg attaatcgtg
     1261 gtctactacc aagcgtgagc cacgtcgccg acgaatttga gcagctctgg ctgccgtact
     1321 ggccgctggc aagcgacgat ctgctcgagg ggatctaccg ccaaagccgc gcgtcggccc
     1381 taggccgccg gtacatcgag gcgaacccaa cagcgctggc aaacctgctg gtcgtggacg
     1441 tagaccatcc agacgcagcg ctccgagcgc tcagcgcccg ggggtcccat ccgctgccca
     1501 acgcgatcgt gggcaatcgc gccaacggcc acgcacacgc agtgtgggca ctcaacgccc
     1561 ctgttccacg caccgaatac gcgcggcgta agccgctcgc atacatggcg gcgtgcgccg
     1621 aaggccttcg gcgcgccgtc gacggcgacc gcagttactc aggcctcatg accaaaaacc
     1681 ccggccacat cgcctgggaa acggaatggc tccactcaga tctctacaca ctcagccaca
     1741 tcgaggccga gctcggcgcg aacatgccac cgccgcgctg gcgtcagcag accacgtaca
     1801 aagcggctcc gacgccgcta gggcggaatt gcgcactgtt cgattccgtc aggttgtggg
     1861 cctatcgtcc cgccctcatg cggatctacc tgccgacccg gaacgtggac ggactcggcc
     1921 gcgcgatcta tgccgagtgc cacgcgcgaa acgccgaatt cccgtgcaac gacgtgtgtc
     1981 ccggaccgct accggacagc gaggtccgcg ccatcgccaa cagcatttgg cgttggatca
     2041 caaccaagtc gcgcatttgg gcggacggga tcgtggtcta cgaggccaca ctcagtgcgc
     2101 gccagtcggc catctcgcgg aagggcgcag cagcgcgcac ggcggcgagc acagttgcgc
     2161 ggcgcgcaaa gtccgcgtca gccatggagg cattgctatg agcgacggct acagcgacgg
     2221 ctacagcgac ggctacaacc ggcagccgac tgtccgcaaa aagcggcgcg tgaccgccgc
     2281 cgaaggcgct cgaatcaccg gactatccga acgccacgtc gtccggctcg tggcgcagga
     2341 acgcagcgag tggctcgccg agcaggctgc acgccgcgaa cgcatccgcg cctatcacga
     2401 cgacgagggc cactcttggc cgcaaacggc caaacatttc gggctgcatc tggacaccgt
     2461 taagcgactc ggctatcggg cgaggaaaga gcgtgcggca gaacaggaag cggctcaaaa
     2521 ggcccacaac gaagccgaca atccaccgct gttctaacgc aattggggag cgggtgtcgc
     2581 gggggttccg tggggggttc cgttgcaacg ggtcggacag gtaaaagtcc tggtagacgc
     2641 tagttttctg gtttgggcca tgcctgtctc gttgcgtgtt tcgttgcgcc cgttttgaat
     2701 accagccaga cgagacgggg ttctacgaat cttggtcgat accaagccat ttccgctgaa
     2761 tatcggggag ctcaccgcca gaatcggtgg ttgtggtgat gtacgtggcg aactccgttg
     2821 tagtgcctgt ggtggcatcc gtggccactc tcgttgcacg gttcgttgtg ccgttacagg
     2881 ccccgttgac agctcaccga acgtagttaa aacatgctgg tcaaactagg tttaccaacg
     2941 atacgagtca gctcatctag ggccagttct aggcgttgtt cgttgcgcgg ttcgttgcgc
     3001 atgtttcgtg tggttgctag atggctccgc aaccacacgc ttcgaggttg agtgcttcca
     3061 gcacgggcgc gatccagaag aacttcgtcg tgcgactgtc ctcgttgatc cttgccgagc
     3121 tgggatggaa gctcggccga ccaccctgga ggagatgatc gaggatgcca gggcctttca
     3181 cgcccgccgc tgctgagcgt ccgccgccgg gcccgcaccg ccgtcggccg gcccgctccg
     3241 ggctcgcagc agcgggcttc ggcgcgggcc cggggctccc gagcgcgggc ggggctccgg
     3301 gcggccgccg ggggccgggg gcggcgccgg gcggcccggg gcgtcaggcg ccgggggcgg
     3361 tgtccggcgg cccccagagg aactgcgcca gttcctccgg atcggtgaag ccggagagat
     3421 ccagcggggt ctcctcgaac acctcgaagt cgtgcaggaa ggtgaaggcg agcagttcgc
     3481 gggcgaagtc ctcggtccgc ttccactgcg ccccgtcgag cagcgcggcc aggatctcgc
     3541 ggtcgccccg gaaggcgttg agatgcagtt gcaccaggct gtagcgggag tctcccgcat
     3601 agacgtcggt gaagtcgacg atcccggtga cctcggtcgc ggccaggtcc acgaagatgt
     3661 tggtcccgtg caggtcgccg tggacgaacc ggggttcgcg gccggccagc agcgtgtcca
     3721 cgtccggcag ccagtcctcc aggcggtcca gcagccgggg cgagaggtag ccccacccgc
     3781 ggtggtcctc gacggtcgcc gcgcggcgtt cccgcagcag ttccgggaag acctcggaat
     3841 ggggggtgag cacggtgttc ccggtcagcg gcaccctgtg cagccggccg agcacccggc
     3901 cgagttcgcg ggccagggcg agcagcgcgt tccggtcggt cgtgccgtcc atcgcggacc
     3961 gccaggtggt gccggtcatc cggctcatca ccaggtaggg ccacggccag gctccggtgc
     4021 cgggccgcag ctcgccgcgg ccgaggaggc ggggcaccgg caccggggcg tccgccagga
     4081 ccgcgtacgc ctccgactcc gacgcgaggc tctccggacc gcaccagtgc tcgccgaaca
     4141 gcttgatcac cgggtcgggc tcgccgacca gtacggggtt ggtgctctcg ccgggcaccc
     4201 gcagcaccgg cggcaccggc agcccgagct cctccagggc tcggcgggcc agcggctccc
     4261 agaattcctg gtcgttccgc aggctcgcgt aggaatcatc cgaatcaata cggtcgagaa
     4321 gtaacaggga ttcttgtgtc acagcggacc tctattcaca gggtacgggc cggcttaatt
     4381 ccgcacggcc ggtcgcgaca cggcctgtcc gcaccgcggt caggcgttga cgatgacggg
     4441 ctggtcggcc acgtccgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4501 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4561 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4621 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4681 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4741 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4801 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     4861 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4921 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4981 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5041 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5101 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5161 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5221 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5281 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5341 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5401 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5461 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5521 aatgcagcta gaactagt
//
