LOCUS Exported 13701 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Thy1-Brainbow-1.1 M SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13701) AUTHORS Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, Lichtman JW TITLE Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. JOURNAL Nature. 2007 Nov 1. 450(7166):56-62. PUBMED 17972876 REFERENCE 2 (bases 1 to 13701) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13701 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(10..28) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 96..200 /gene="bla" /label=AmpR promoter CDS 201..1061 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(419..438) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1232..1820 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1721..1740 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1974..1991 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6253..6286 /label=loxN /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GTATACCT). loxN sites are compatible with each other, but incompatible with loxP or lox2272 sites." protein_bind 6294..6327 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." protein_bind 6337..6370 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." regulatory 6384..6393 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6390..7046 /codon_start=1 /product="Kusabira-Orange fluorescent protein (Karasawa et al., 2004)" /label=Kusabira-Orange /translation="MVSVIKPEMKMKYFMDGSVNGHEFTVEGEGTGKPYEGHQEMTLRV TMAKGGPMPFSFDLVSHTFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLQFEDGGFA AVSAHISLRGNCFEHKSKFVGVNFPADGPVMQNQSSDWEPSTEKITTCDGVLKGDVTMY LKLAGGGNHKCQFKTTYKAAKKILKMPQSHFIGHRLVRKTEGNITELVEDAVAHC" polyA_signal 7169..7250 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind 7288..7321 /label=loxN /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GTATACCT). loxN sites are compatible with each other, but incompatible with loxP or lox2272 sites." regulatory 7442..7451 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 7448..8158 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(7593..7611) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 7861..7880 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 8286..8407 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind 8490..8523 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." regulatory 8642..8651 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 8648..9367 /codon_start=1 /product="enhanced YFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" polyA_signal 9490..9611 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind 9626..9659 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." regulatory 9782..9791 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 9788..10507 /codon_start=1 /product="enhanced monomeric variant of CFP (Rizzo et al., 2004)" /label=mCerulean /note=" mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" protein_bind 10550..10583 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" polyA_signal 10607..10728 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(13532..13551) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 13651..13673 /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 2041 cgattcatta atgcaggatc gcggccgcga tccccgggcg agctcgaatt cagagaccgg 2101 gaaccaaact agcctttaaa aaacataagt acaggagcca gcaagatggc tcagtgggta 2161 aaggtgccta ccagcaagcc tgacagcctg agttcagtcc ccacgaacta cgtggtagga 2221 gaggaccaac caactctgga aatctgttct gcaaacacat gctcacacac acacacacaa 2281 atagtataaa caattttaaa tttcatttaa aaataatttg taaacaaaat cattagcaca 2341 ggttttagaa agagcctctt ggtgacatca agttgatgct gtagatgggg tatcattcct 2401 gaggacccaa aaccgggtct cagcctttcc ccattctgag agttctctct tttctcagcc 2461 actagctgaa gagtagagtg gctcagcact gggctcttga gttcccaagt cctacaactg 2521 gtcagcctga ctactaacca gccatgaaga aacaaggagt ggatgggctg agtctgctgg 2581 gatgggagtg gagttagtaa gtggccatgg atgtaatgac cccagcaatg ctggctagaa 2641 ggcatgcctc ctttccttgt ctggagacgg aacgggatca tcttgtactc acagaaggga 2701 gaacattcta gctggttggg ccaaaatgtg caagttcacc tggaggtggt ggtgcatgct 2761 tttaactcca gtactcagga ggcagggcca ggtggatctc tgtgagttca agaccagcct 2821 gcactatgga gagagttttg ggacagccag agttacacag aaaaatcctg gtggaaaatc 2881 tgaaagaaag agagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa 2941 agaaagaaag aaagaaagga agaaagaaag aaagagtggc aggcaggcag gcaggaggaa 3001 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa 3061 aataggtgcg acttcaagat ccggagttac aagcagaatg cactgtttcc ctaacagggc 3121 caagtgtttt gagtaactga aggtgggcat gatgcctggg aagcagaaac aagccaggca 3181 gatgcacccc ttgccttgct tccgaagggc tgcagtagca tggaaaacat ggaaaacaac 3241 caatccattc cctttgctga tataacaggc tccaaagcca aaacctgtca ctggaggctc 3301 aagagcagat ctccagccaa gaggcaaagg aatgggggaa gctggagggc ctccctctgg 3361 ttatccaggc ttctgaaggt tcaagcaaag aaagggttac aaccttaaaa ggagagcgtc 3421 ccggggtatg ggtagaagac tgctccaccc cgacccccag ggtccctaac cgtcttttcc 3481 ctgggcgagt cagcccaatc acaggactga gagtgcctct ttagtagcag caagccactt 3541 cggacaccca aatggaacac ctccagtcag ccctcgccga ccaccccacc ccctccatcc 3601 ttttccctca gcctccgatt ggctgaatct agagtccctc cctgctcccc cctctctccc 3661 cacccctggt aaaactgcgg gcttcaggct gggtgcagca actggaggcg ttggcgacca 3721 ggaggaggct gcagctaggg gagtccaggt gagagcaggc cgacgggagg gacccgcaca 3781 tgcaaggacc gccgcagggc gaggatgcaa gccttcccca gctacagttt tgggaaagga 3841 taccagggcg ctcctatatg ggggcgcggg aacctgggga aagaaggtgc tcccaggtcg 3901 aggtgggaga ggaaggcagt gcggggtcac gggctttctc cctgctaacg gacgctttcg 3961 aagagtgggt gccggagaga accatgagga aggacatcaa ggacagcctt tggtccccaa 4021 gctcaaatcg ctttagtggt gcgaatagag ggaggaggtg ggtggcaaac tggagggagt 4081 ccccagcggt gacctcgtgg ctggctggtg cggggcacca ggtaagaaac gcaatgttgc 4141 gggaggggac tgggtggcag gcgcggggga ggggaaagct agaaaggatg cgagggagcg 4201 gaggggggag ggagcggggg aatctcaact ggtagaggaa agttaaaatg aggaaatagc 4261 atcagggtgg ggttagccaa gccgggcctc agggaaaggg cgcaaagttt gtctgggtgt 4321 gggcttaggt gggctgggta tgagattcgg ggcgccgaaa acactgctgc gcctctgcca 4381 aatcacgcta cccctgtatc tagttctgct aggcttctcc agccccagcc ccaattcttt 4441 tctcagtgtc cccttccctc ccctgaatct caagcccaca ctccctcctc cataacccac 4501 tgttatcaaa tctaagtcat ttgccaccca acaaccatca ggaggcggaa gcagacggga 4561 ggagtttgag atcaacttgg gctacatcac gagttccaag ctcaccaagg cttcttaagg 4621 agaccttgtc tctaaaatta attaattaat taattaatag tcccctttct ctgccacaga 4681 accttgggat ctggctcctg gtcgcagctc cccccacccc aggctgacat tcactgccat 4741 agcccatccg gaaatcctag tctattcccc atggatcttg aactgcagag agaatggcag 4801 agtggcccgc cctgtgcaaa ggatgttcct agcctaggtg gagctcgcga actcggagac 4861 tgtgcctctc ttgggcaagg acaggctaga cagcctgccg gtgttgagct agggcactgt 4921 ggggaaggca gagaacctgt gcagggcacg caatgaacac aggaccagaa aactgcagcc 4981 ctaggaacac tcaagagctg gccatttgca agcatctctg gcctccgtgc ttctcactca 5041 tgtcccatgt cttatacagg cctctgtggc acctcgcttg cctgatctca tccctagccg 5101 ttaagctttc tgcatgactt atcacttggg gcataatgct ggatacctac cattttctta 5161 gaccccatca aaatcctatt tgagtgtacg gttcggagaa ctccttattt atccggtaaa 5221 tgtcttttac tctgctctca gggagctgag gcaggacatt cctgagatac attgggagag 5281 gaatacagtt tcaataaaat aataggttgg gtggaggtac atgcctataa tgccaccact 5341 caggaaatgg tggcagcttc gtgagtttga ggccaaccca agaaacatag tgaaaccctg 5401 tcagtaaata agtaagcaag tatttgagta tctactatat gctagggctg acctggacat 5461 taggggtcat cttctgaaca aactagtgct tgagggaggt atttggggtt tttgtttgtt 5521 taatggatct gaatgagttc cagagactgg ctacacagcg atatgactga gcttaacacc 5581 cctaaagcat acagtcagac caattagaca ataaaaggta tgtatagctt accaaataaa 5641 aaaattgtat tttcaagaga gtgtctgtct gtgtagccct ggctgttctt gaactcactc 5701 tgtagaccag gctggcctgg aaatccatct gcctctgcct ctctgcctct ctgcctctct 5761 gcctctctct ctgcctctct ctgcctctct ctgcccctct ctgcccctct ctgcccctct 5821 ctgccgccct ctgccttttg ccctctgccc tctgttctct ggcctctgcc ctctccctgc 5881 cttctccctc tgcctctgcc tcttgagtgc tggaatcaaa ggtgtgagct ctataggtct 5941 taagttccag aagaaacgta atgaagtcac ccagcaggag gtgctcaggg acagcaagac 6001 acacacaccc aggacactag gctcccactt ccttggcttt ctctgagtgg caaaggacct 6061 taggcagtgt cactccctaa gagaagggga taaagagagg ggctgaggta ttcagtcatg 6121 tgctccgtgg atctcaagcc ctcaaggtaa atggggaccc acctgtccta ccagctggct 6181 gacctgtagc tttccccacc acagaatcca agtcggaact cttggcacct agaggatctc 6241 gactagctcg agataacttc gtatagtata ccttatacga agttatcgag ctcataactt 6301 cgtataggat actttatacg aagttataac gtcgagataa cttcgtatag catacattat 6361 acgaagttat accggcagat ctcgccacca tggtgagtgt gattaaacca gagatgaaga 6421 tgaagtactt catggacggc tccgtcaatg ggcatgagtt cacagttgaa ggtgaaggca 6481 caggcaaacc ttacgaggga catcaagaga tgacactacg cgtcacaatg gccaagggcg 6541 ggccaatgcc tttctcgttt gacttagtgt cacacacgtt ctgttacggc cacagacctt 6601 ttactaaata tccagaagag ataccagact atttcaaaca agcatttcct gaaggcctgt 6661 catgggaaag gtcgttgcag ttcgaagatg gtgggttcgc tgccgtcagt gcgcatataa 6721 gccttagagg aaactgcttc gagcacaaat ccaaatttgt tggggttaac tttcctgccg 6781 atggtcctgt gatgcaaaac caaagttctg attgggagcc atcaaccgag aaaattacta 6841 cctgcgacgg agttctgaag ggtgatgtta cgatgtacct aaaacttgcg ggaggcggca 6901 atcacaaatg ccaattcaag actacttaca aggcggcaaa aaagattctt aaaatgccac 6961 aaagccattt catcgggcat cgcctcgtca ggaaaaccga aggcaacatt actgagctgg 7021 tagaagatgc agtagctcat tgctgaagcg gccgcgactc tagatcataa tcagccatac 7081 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa 7141 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa 7201 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 7261 tggtttgtcc aaactcatca atgtatcata acttcgtata gtatacctta tacgaagtta 7321 taggatctcc gccaccatgc tgtgctgcat cagaagaact aaaccggttg agaagaatga 7381 agaggccgat caggagctgc agtcgacggt accgcgggcc cgggatccac cggtagcatc 7441 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg 7501 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg 7561 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc 7621 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt 7681 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg 7741 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct 7801 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg 7861 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga 7921 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta 7981 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta 8041 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca 8101 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaac 8161 acgtgcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg 8221 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 8281 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 8341 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 8401 tatcttaagg cgtaaattgt aagcgttaat gctccgaggg ccgccaccac ctgttcctgt 8461 aggaattggc cgcgactcta ggatctagga taacttcgta taggatactt tatacgaagt 8521 tattgatcaa ttcacagcca ccatgctgtg ctgcatcaga agaactaaac cggttgagaa 8581 gaatgaagag gccgatcagg agctgcagtc gacggtaccg cgggcccggg atccaccggt 8641 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 8701 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 8761 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 8821 gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca 8881 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 8941 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 9001 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 9061 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 9121 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 9181 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 9241 caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca 9301 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 9361 caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta 9421 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt 9481 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 9541 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 9601 aatgtatctt aacgcgcgat tcgagataac ttcgtatagc atacattata cgaagttata 9661 ccggaattaa ttcacagcca ccatgctgtg ctgcatcaga agaactaaac cggttgagaa 9721 gaatgaagag gccgatcagg agctgcagtc gacggtaccg cgggcccggg atccaccggt 9781 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 9841 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 9901 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 9961 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca 10021 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 10081 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 10141 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 10201 ggggcacaag ctggagtaca acgccatcag cgacaacgtc tatatcaccg ccgacaagca 10261 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca 10321 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 10381 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca 10441 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 10501 caagtaaagc ggccgcgact ctagatcacc gcgactctag atcacgcgcg aagttcctat 10561 tctctagaaa gtataggaac ttctcgagta ctcaattgtt gttgttaact tgtttattgc 10621 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 10681 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg 10741 taagcgttaa ttcgaggtcc ttcctctgca gaggtcttgc ttctcccggt cagctgactc 10801 cctccccaag tccttcaaat atctcagaac atggggagaa acggggacct tgtccctcct 10861 aaggaacccc agtgctgcat gccatcatcc cccccaccct cgcccccacc cccgccactt 10921 ctccctccat gcataccact agctgtcatt ttgtactctg tatttattct agggctgctt 10981 ctgattattt agtttgttct ttccctggag acctgttaga acataagggc gtatggtggg 11041 taggggaggc aggatatcag tcccctgggg cgagttcctc cctgccaacc aagccagatg 11101 cctgaaagag atatggatga gggaagttgg actgtgcctg tacctggtac agtcatactc 11161 tgttgaaaga atcatcgggg aggggggggg gctcaagagg ggagagctct ctgagccttt 11221 gtggaccatc caatgaggat gagggcttag attctaccag gtcattctca gccaccacac 11281 acaagcgctc tgccatcact gaagaagccc cctagggctc ttgggccagg gcacactcag 11341 taaagatgca ggttcagtca gggaatgatg gggaaagggg taggaggtgg gggagggatc 11401 accccctcct ctaaaacacg agcctgctgt ctccaaaggc ctctgcctgt agtgagggtg 11461 gcagaagaag acaaggagcc agaactctga ctccaggatc taagtccgtg caggaagggg 11521 atcctagaac catctggttg gacccagctt accaagggag agcctttatt ccttctttcc 11581 cttgcccctc tgtgccagcc cctcttgctg tccctgatcc cccagacagc gagagtcttg 11641 caacctgcct cttccaagac ctcctaatct caggggcagg cggtggagtg agatccggcg 11701 tgcacacttt ttggaagata gctttcccaa ggatcctctc ccccactggc agctctgcct 11761 gtcccatcac catgtataat accaccactg ctacagcatc tcaccgagga aagaaaactg 11821 cacaataaaa ccaagcctct ggagtgtgtc ctggtgtctg tctcttctgt gtcctggcgt 11881 ctgtctcttc tgtgttcttc caaggtcaga aacaaaaacc acacacttca acctggatgg 11941 ctcggctgag cacttctgtg tgcagaaggt ccaaccagac tctggggtac cccggccctc 12001 cctattccct tgcctcctgt ctcccgcttt ttatagctcc ctatgctggg cttctctgga 12061 gagtgaaatc tttgcccaaa tcaatgcgca ttctctctgc tgagtcatct ggcgacagca 12121 gttgagttca cccgccaaca catgggccca gctatgtagc cgaaccctgg ctctggaagt 12181 gccagggact ttgtgcataa gtatgtacca tgcccttttt tcacagtcct agctctgcag 12241 aagtgcagcc tgaaggcctg tctgctgaga ggacatgccc tggagccctg aaacaggcac 12301 agtgggagga ggaacggagg atgacaggca tcaggccctc agtccaaaag caaccacttg 12361 agaatgggct ggagtacgaa acatggggtc ccgtccctgg atccctcctc aaagagtaat 12421 aagtaaaata taaacaggta ccccaggccg ttctgggttt gggttgtaat gggatccatt 12481 tgcagagaac tattgagaca gcccagccgt actgtgacag gcaatgtggg ggaggaggtt 12541 gaatcacttg gtatttagca tgaatagaat aattccctga acatttttct taaacatcca 12601 tatctaaatt accaccactc gctcccagtc ttcctgcctt tgcgccagcc tcctgtctgg 12661 ccatgcctga agaaggctgg agaagccacc cacctcaggc catgacactg ccagccactt 12721 ggcaggtgca gccaaacctg agctgtccca gaaagggaca ttctcaagac ccaggcaccc 12781 tgatcagcac tgacttggag ctacaagtgt catgccagaa aagtctctaa gaaaaccttt 12841 tcagggaaaa gggggtgact caacaccggg caagtttggg aagccccacc cttcgagtga 12901 tggaagagca gataggaagc ctcagaagag agacaccggc acccaggtaa cgttcctcat 12961 gtggtctctg tcacactagg tgctcttccc tggacatctc cgtgaccaca ctctcagttc 13021 ttagggagat gcgggtgctc tctgaggcta tctcagagtt gcagattctg aggcctagag 13081 tgactacagt cagcctagga agccacagag gactgtggac caggagggca gaagaggaga 13141 agggaagaaa aaccatcaga taggacttgc aatgaaacta acccaagaca atcataatgc 13201 agacaggaat gttaaaggcg ttcagcagct ggccatgaca cccatctgtc cctctggcca 13261 agtcagcaag cctggaagac ctgggactcc tgcccatatg tcctaagctc cccacccacc 13321 cactcgttca ctgtccttat tctctctcta ccttcagcca cttagtttcc taccttaagt 13381 cctagaattg atcctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 13441 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg 13501 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 13561 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 13621 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 13681 cgtcatcacc gaaacgcgcg a //