LOCUS       Exported                3507 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    mEos4b-CAF1-C-10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3507)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      26..35
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             32..712
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     protein_bind    2531..2542
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagt gcgattaagc cagacatgag
       61 gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg
      121 tacaggcaag ccttatgagg gaaaacagac catggatctt gaagtcaaag agggcggacc
      181 tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca gggtattcgt
      241 gaaatatcca gacaacatac aagactattt taagcagtcg tttcctaagg ggtattcgtg
      301 ggaacgaagc ttgactttcg aagacggggg catttgcaat gccagaaacg acataacaat
      361 ggaaggggac actttctata ataaagttcg attttatggt accaactttc ccgccaatgg
      421 tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg
      481 tgatggagtg ctgacgggtg atattgagat ggctttgttg cttgaaggaa atgcccatta
      541 ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcgc
      601 ccactttgtg gaccacgcca ttgagatttt aagccatgac aaagattaca acaaggttaa
      661 gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac gatccggact
      721 cagatctggc agcggtggag gcttggagga gccggaggcc gcgacgcgga cagccgcggc
      781 cgtggattgc aaagacagac ccgggttccc agtgaagagg ttaatacaag cccgcttgcc
      841 cttcaaacgc ctgaaccttg ttcccaagga gaaagttgag gaagacacat caccaaaagc
      901 agctgtggaa agcaaagtcc cggatctaca attgtctttg ggcacttttg aaagccagtg
      961 tcacacaggc tctcacgtag gcttgagtac aaagttggtc ggtggccagg gccccattga
     1021 tagcttcttg agggcaacaa tcaagccagt accgagtgtg gtcattatcg acctgacgga
     1081 gaactgcagt gacattccag acagccctga gggccacagt gaactaagtc ctgacactgc
     1141 tggtgtggtc accacagtgg aaggagctgc caagcagcag gagcactctg cagcagagct
     1201 gtgtctccta gagacccctt cagacatcac ttgtcacatg gaagaggagc ctgggagtcc
     1261 aggagaccca aagaggacgg gtgactgtca ggctggttca ctacagagct gtccggagct
     1321 gaccccaggt tctaggacat gccccaccaa ggagttatca agctggagca aggcagggga
     1381 tctcctattt atagagaaag tgcctgtggt ggtgctagag gacatcctgg ccactaagcc
     1441 ctccattgcc tctcttccca tgatgtccct ggataggagt gtcacttcag agagtgaaat
     1501 actggagtcc tgccccgagg atgactccat actgagccac tcctccacca actcctcttc
     1561 tccaaccagc tctcctgagg gaccatccac acccccagaa catcggggtg ggagaagctc
     1621 cccctccacc ccagcctgca gagttgccaa gaactttgtc aaaggctcca cagagaaggg
     1681 caggagcaaa ctgcacagag atagagaaca gcagagggaa gagaaggaaa agctgaggga
     1741 agagatacga cgagccaagg aggaggcacg gaagaagaag gaggaggaga aggagctgaa
     1801 ggaaaaggag cggagggaaa agcgggagaa agatgagaag gagaaggcgg agaagcagcg
     1861 gctaaaggag gaaaaacgca aggagcggca ggaagctctg gaggctaaac tggaggagaa
     1921 gaggaagaag gaggaagaga agcggttacg ggaggaagag aagcggttac gggaagaaga
     1981 gaagcgcatt aaggcagaaa aggcagagat cactcgattc ttccagaaac cgaagactcc
     2041 gcaggctccc aagaccctgg ccggttcctg tggtaagttt gccccctttg aaatcaaaga
     2101 acacatggtg ctggctccac ggtgccgagc tgccttggat caagacctgt gcgatcagct
     2161 ggaccagctc ctgcagcagc agagtgtggc aagcaccttc ctcagtgacc taaaaagccg
     2221 gctgcccttg agatcggggc ccacccgggt ctgtggccat gacacagaca tcatgaacag
     2281 ggatgtggtc attgtggaga gcagcaaggt ggacggtgtg tctgaaagga agaagtttgg
     2341 ccgcatgaag cttctgcagt tctcagagaa ccaccggcca gcatactggg ggacgtggaa
     2401 caagaagaca gccatcatcc gcccccggaa cccctgggcc caggacaaag accttcttga
     2461 ctatgaggta gacagtgacg atgagtggga agaggaggaa cctggggagt ctttgtccca
     2521 cagtgagggg gatgaggatg acgatgtggg ggaagatgag gatgaggacg atggcttctt
     2581 tgtgccccat gggtacttat ctgaagatga aggcgtgact gaggagtgtg cagaccctga
     2641 gaaccacaaa gtacaccaga agctgaaggc caaggagtgg gatgagctcc tagccaaggg
     2701 caagcgcttt cgtgtgctgc agcctgtgca cgtgggatgt gtatgggcat cagaggcagc
     2761 caactgcacg agttccgact tgaagctgct gcagcagttc actgcatgcc tgctggatgt
     2821 ggcatcccct gatgagccag aacccggggc ctccaggagg gaaaagaggg accagcatat
     2881 cctggcccag ttgcttccgc tgctacatgg caatgtgaat gggagcaagg tgatcatcca
     2941 tgaattccag gagcaatgcc gcaggggcct gctgaccctt ccaagtccca ccccacacct
     3001 gcagatgccc aacttggagg atgctgttgc tgtgccatcc aaggccaggc tgaagcgcct
     3061 tatctctgag aactctgcct atgagaagcg gcccaacttc cgtatgtgct ggtatgtgca
     3121 cccagaggtg ctcaagagct ttggccagga gtgcctgcca gtgccctgcc agtggaccta
     3181 catcaccacc atgccctcag cacccaggga agacagtggc agtgcttcta ctgaggggcc
     3241 tggccagagc acacctatgc tactcaagcg gaagccagcg gccaccatgt gcatcacgca
     3301 gtttatgaag aagcggcgct atgatggaca ggttgggtct ggggacatgg atggcttcca
     3361 ggcagacaca gaagaggatg aagaggatga cacagactgt atgatcatag atgtgccaga
     3421 tgttgggagt gacgtgtcag aagctcccat acctgctccc acactttgca agtgaggatc
     3481 caccggatct agataactga tcataat
//
