LOCUS Exported 7399 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS ODD-Luciferase-pcDNA3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7399) AUTHORS Safran M, Kim WY, O'Connell F, Flippin L, Gunzler V, Horner JW, Depinho RA, Kaelin WG TITLE Mouse model for noninvasive imaging of HIF prolyl hydroxylase activity: assessment of an oral agent that stimulates erythropoietin production. JOURNAL Proc Natl Acad Sci U S A. 2006 Jan 3. 103(1):105-10. PUBMED 16373502 REFERENCE 2 (bases 1 to 7399) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7399 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 907..916 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1285..2934 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF VDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(1348..1366) /label=LucNrev /note="Firefly luciferase, reverse primer" promoter complement(2951..2969) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(2952..2969) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(2989..3006) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2995..3219 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 3265..3693 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3352..3371) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3562..3583 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3702..3722) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3707..4037 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3888..4023 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3950..3969 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4104..4898 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4158..4177) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4768..4787 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 5072..5193 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5109..5128) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5163..5182 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(5242..5258) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5242..5258) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5255..5277) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5266..5282 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5290..5320) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5335..5356 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5473..5490) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5644..6232) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5724..5743) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6403..7263) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7026..7045 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7264..7368) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggatcc 901 gaattcgcca ccatggaatt caagttggaa ttggtagaaa aactttttgc tgaagacaca 961 gaagcaaaga acccattttc tactcaggac acagatttag acttggagat gttagctccc 1021 tatatcccaa tggatgatga cttccagtta cgttccttcg atcagttgtc accattagaa 1081 agcagttccg caagccctga aagcgcaagt cctcaaagca cagttacagt attccagcag 1141 actcaaatac aagaacctac tgctaatgcc accactacca ctgccaccac tgatgaatta 1201 aaaacagtga caaaagaccg tatggaagac attaaaatat tgattgcatc tccatctcct 1261 acccacatac ataaagaaac tactgaagac gccaaaaaca taaagaaagg cccggcgcca 1321 ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac 1381 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac 1441 gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat 1501 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg 1561 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa 1621 ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg 1681 caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat 1741 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct 1801 cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca 1861 ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga 1921 actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg 1981 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc 2041 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt 2101 ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc 2161 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct 2221 tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg 2281 ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct gattacaccc 2341 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt 2401 gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga 2461 ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac 2521 aaggatggat ggctacattc tggagacata gcttactggg acgaagacga acacttcttc 2581 atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa 2641 ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc 2701 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg 2761 acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc 2821 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga 2881 aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt gtaattctag 2941 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc 3001 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 3061 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 3121 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 3181 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 3241 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 3301 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 3361 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 3421 gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 3481 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 3541 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 3601 tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa 3661 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 3721 gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt 3781 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 3841 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 3901 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 3961 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 4021 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 4081 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 4141 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 4201 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 4261 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 4321 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 4381 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 4441 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 4501 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 4561 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 4621 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 4681 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 4741 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 4801 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 4861 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 4921 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 4981 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 5041 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 5101 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 5161 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 5221 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 5281 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 5341 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 5401 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 5461 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 5521 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 5581 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 5641 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 5701 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 5761 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 5821 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg 5881 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 5941 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 6001 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 6061 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 6121 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 6181 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 6241 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 6301 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 6361 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 6421 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 6481 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 6541 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 6601 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 6661 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 6721 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 6781 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 6841 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 6901 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 6961 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 7021 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 7081 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 7141 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 7201 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 7261 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 7321 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 7381 aaaagtgcca cctgacgtc //