LOCUS       Exported                1048 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Fluid Shear Stress inducible Red Fluorescent Protein for mammalian 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    EGR-1 TurboRFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Varma S, Voldman J
  TITLE     A cell-based sensor of fluid shear stress for microfluidics.
  JOURNAL   Lab Chip. 2015 Feb 4.
  PUBMED    25648195
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(146..841)
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct agagtcgcgg ccgcatcatc tgtgccccag tttgctaggg aggtcgcagt
      181 acttggccac agccatctcg tgctgctcga cgtaggtctc tttgtcggcc tccttgattc
      241 tttccagtct gtggtccacg aagtggaagc cgggcatctt gaggttctta gcgggtttct
      301 tggatctgta tgtggtcttg aaggagcagt gcaggtagcc cccgcccacg agcttcaggg
      361 ccatctggct gtggcctctc aggccgccgt cagcggggta cagcatctcg gtgttggcct
      421 cccagccgcg tgttttcttc tgcatcacag ggccgttgga tgggaagttc accccgttga
      481 tcttgacgtt gtagatgatg cagccgttct ggaagctggt gtcctgggta gcggtcagca
      541 cgcccccgtc ttcgtatgtg gtgattctct cccatgtgaa gccctcaggg aaggactgct
      601 taaagaagtc ggggatgccc tgggtgtggt tgatgaaggc tttgctgccg tacatgaagc
      661 tggtagccag gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgatcttca
      721 tggtctgggt gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtggtggt
      781 tgttcacggt gccctccatg tacagcttca tgtgcatgtt ctccttgatc agctcgctca
      841 tggtggcgac cggtgcgact ccccgaatcg gcctctattt caagggtctg gaacagcacg
      901 ggcccgcctc cgccgtgacg tacatggcca tatatgggaa gcaggaagcc ctaatatgga
      961 aggaccggcc gggaggaccc ggagtgacgt gaagccctcc catccaagag tggtgggcac
     1021 ctccaccctg cgacccgctc cggcatcg
//
