LOCUS Exported 171 bp ds-DNA linear SYN 06-JUN-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS PL-452 C-Flag-4C SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 171) AUTHORS Venken KJ, Kasprowicz J, Kuenen S, Yan J, Hassan BA, Verstreken P TITLE Recombineering-mediated tagging of Drosophila genomic constructs for in vivo localization and acute protein inactivation. JOURNAL Nucleic Acids Res. 2008 Aug 1. ():. PUBMED 18676454 REFERENCE 2 (bases 1 to 171) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 6, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..171 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(37..70) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS 94..117 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 142..159 /codon_start=1 /product="tetracysteine peptide that binds biarsenical labeling reagents" /label=tetracysteine tag /translation="CCPGCC" ORIGIN 1 gcagcccaat tccgatcata ttcaataacc cttaatataa cttcgtataa tgtatgctat 61 acgaagttat taggtccctc gaggggatcc atggattaca aggatgacga cgataagggc 121 tccgccggat cgtttttgaa ttgctgcccc ggctgctgca tggagcccta a //