LOCUS Exported 3675 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMOD4 galK-GT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3675) AUTHORS Zhang Y, Nash L, Fisher AL. TITLE A simplified, robust, and streamlined procedure for the production of C. elegans transgenes via recombineering JOURNAL BMC Dev Biol. 2008 Dec 30;8(1):119. PUBMED 19116030 REFERENCE 2 (bases 1 to 3675) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3675 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 124..147 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" promoter 196..243 /label=EM7 promoter /note="synthetic bacterial promoter " misc_feature 1677..1695 /label=Tn5 ME /note="hyperactive mosaic end for Tn5 transposase recognition (Reznikoff et al., 2004)" primer_bind complement(1834..1853) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1953..1975 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(2013..2031) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2099..2203 /gene="bla" /label=AmpR promoter CDS 2204..3064 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2422..2441) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" primer_bind 3522..3539 /label=L4440 /note="L4440 vector, forward primer" misc_feature 3605..3623 /label=Tn5 ME /note="hyperactive mosaic end for Tn5 transposase recognition (Reznikoff et al., 2004)" ORIGIN 1 ctgcagatgg caggccttgc gcaacacgat gaagccgtgg acaacaaatt caacaaaacc 61 ggtatggaaa agagaagatg gaaaaagaat ttcatagccg tctcagcagc caactctaga 121 atggattaca aggacgatga cgataagatg agtaaaggag aagaactttt cactggagtt 181 gtcccaagaa ttcctgttga caattaatca tcggcatagt atatcggcat agtataatac 241 gacaaggtga ggaactaaac ccaggaggca gatcatgagt ctgaaagaaa aaacacaatc 301 tctgtttgcc aacgcatttg gctaccctgc cactcacacc attcaggcgc ctggccgcgt 361 gaatttgatt ggtgaacaca ccgactacaa cgacggtttc gttctgccct gcgcgattga 421 ttatcaaacc gtgatcagtt gtgcaccacg cgatgaccgt aaagttcgcg tgatggcagc 481 cgattatgaa aatcagctcg acgagttttc cctcgatgcg cccattgtcg cacatgaaaa 541 ctatcaatgg gctaactacg ttcgtggcgt ggtgaaacat ctgcaactgc gtaacaacag 601 cttcggcggc gtggacatgg tgatcagcgg caatgtgccg cagggtgccg ggttaagttc 661 ttccgcttca ctggaagtcg cggtcggaac cgtattgcag cagctttatc atctgccgct 721 ggacggcgca caaatcgcgc ttaacggtca ggaagcagaa aaccagtttg taggctgtaa 781 ctgcgggatc atggatcagc taatttccgc gctcggcaag aaagatcatg ccttgctgat 841 cgattgccgc tcactgggga ccaaagcagt ttccatgccc aaaggtgtgg ctgtcgtcat 901 catcaacagt aacttcaaac gtaccctggt tggcagcgaa tacaacaccc gtcgtgaaca 961 gtgcgaaacc ggtgcgcgtt tcttccagca gccagccctg cgtgatgtca ccattgaaga 1021 gttcaacgct gttgcgcatg aactggaccc gatcgtggca aaacgcgtgc gtcatatact 1081 gactgaaaac gcccgcaccg ttgaagctgc cagcgcgctg gagcaaggcg acctgaaacg 1141 tatgggcgag ttgatggcgg agtctcatgc ctctatgcgc gatgatttcg aaatcaccgt 1201 gccgcaaatt gacactctgg tagaaatcgt caaagctgtg attggcgaca aaggtggcgt 1261 acgcatgacc ggcggcggat ttggcggctg tatcgtcgcg ctgatcccgg aagagctggt 1321 gcctgccgta cagcaagctg tcgctgaaca atatgaagca aaaacaggta ttaaagagac 1381 tttttacgtt tgtaaaccat cacaaggagc aggacagtgc tgaggatccc ttcttgagtt 1441 tgtaacagct gctgggatta cacatggcat ggacgaacta tacaaaagcc cacaagcttt 1501 gggtacccta aatggtgctc aggcgccgaa agtagacgcg aattccgcgg ggaagtcaac 1561 cagtactgcc gtctcagcag ccaaccgctt taagaaaatc tcatcctccg gggcacttga 1621 gctcgaattc gagccaatat gcgagaacac ccgagaaaat tcatcgatga tggttgagat 1681 gtgtataaga gacagctgtc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 1741 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg 1801 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 1861 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc 1921 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc 1981 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt 2041 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 2101 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 2161 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 2221 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 2281 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 2341 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 2401 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 2461 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 2521 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 2581 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 2641 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 2701 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 2761 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 2821 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 2881 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 2941 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 3001 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 3061 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 3121 atttgtgcat ccgaagatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt 3181 ttcacgtact aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc 3241 tcatggctaa cgtactaagc tctcatggct aacgtactaa gctctcatgg ctaacgtact 3301 aagctctcat gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg 3361 ttttaagttt tataagaaaa aaaagaatat ataaggcttt taaagctagc ttttaaggtt 3421 tcaccatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 3481 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 3541 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaag 3601 acagctgtct cttatacaca tctcaaccct gaagctcttg ttggctagtg cgtagtcgtt 3661 ggcaagcttg catgc //