LOCUS Exported 7286 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Dominant negative Dynamin 1 mutant tagged with mRFP. ACCESSION . VERSION . KEYWORDS Dyn1(K44A)-mRFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7286) AUTHORS Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH TITLE Live cell imaging of Gs and the beta2-adrenergic receptor demonstrates that both alphas and beta1gamma7 internalize upon stimulation and exhibit similar trafficking patterns that differ from that of the beta2-adrenergic receptor. JOURNAL J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5. PUBMED 15297467 REFERENCE 2 (bases 1 to 7286) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7286 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 197..500 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 501..704 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 655..675 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 3404..3413 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3410..4087 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /note="mammalian codon-optimized" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" primer_bind complement(3540..3558) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 3808..3827 /label=mCherry-F /note="mCherry, forward primer" primer_bind 4002..4025 /label=DsRed1-C /note="DsRed1, forward primer" polyA_signal 4210..4331 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4247..4266) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4301..4320 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(4338..4793) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4475..4496) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4687..4706 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4820..4924 /gene="bla" /label=AmpR promoter promoter 4926..5283 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(4949..4969) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 5134..5269 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5196..5215 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5318..6112 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5372..5391) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5982..6001 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(6300..6319) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 6344..6391 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 6720..22 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7209..7228 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 61 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 121 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc 181 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 241 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 301 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 361 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 421 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 481 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 541 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 601 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 661 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc 721 agatccgcta gcgctaccgg actcagatcc tgccatgggc aaccgcggca tggaagatct 781 catcccgctg gtcaaccggc tgcaagacgc cttctctgcc atcggccaga acgcggacct 841 cgacctgccg cagatcgctg tggtgggcgg ccagagcgcc ggcgcgagct cggtgctcga 901 gaatttcgta ggcagggact tcttgcctcg aggatctggc attgttaccc gacgtcccct 961 ggtcttgcag ctggtcaatg caaccacaga atatgccgag ttcctgcact gcaagggaaa 1021 gaaattcacc gacttcgagg aggtgcgcct tgagatcgag gccgagaccg acagggtcac 1081 cggcaccaac aagggcatct cgccggtgcc tatcaacctc cgcgtctact cgccgcacgt 1141 gctgaacctg accctggtgg acctgcccgg aatgaccaag gtcccggtgg gggaccaacc 1201 tcccgacatc gagttccaga tccgagacat gcttatgcag tttgtcacca aggagaactg 1261 cctcatcctg gccgtgtccc ccgccaactc tgacctggcc aattctgacg ccctcaaggt 1321 cgccaaggag gtggaccccc agggccagcg caccatcggg gtcatcacca agctggacct 1381 gatggacgag ggcacagatg cccgtgatgt gctggagaac aagctgctcc ccctgcgcag 1441 aggctacatt ggagtggtga accggagcca gaaggacatt gatggcaaga aggacattac 1501 cgccgccttg gctgctgaac gaaagttctt cctctcccat ccatcttatc gccacttggc 1561 tgaccgtatg ggcacgccct acctgcagaa ggtcctcaat cagcaactga cgaaccacat 1621 ccgggacaca ctgccggggc tgcggaacaa gctgcagagc cagctactgt ccattgagaa 1681 ggaggtggag gaatacaaga acttccgccc tgatgaccca gctcgcaaga ccaaggccct 1741 gctgcagatg gtccagcagt ttgccgtaga ctttgagaag cgcattgagg gctcaggaga 1801 tcagatcgac acctacgaac tgtcaggggg agcccgcatt aaccgaatct tccacgagcg 1861 cttccctttc gagctggtca agatggagtt tgatgagaag gaactccgaa gggagatcag 1921 ctatgctatc aagaatatcc atggcattag aacggggctg tttaccccag acatggcctt 1981 tgagaccatt gtgaaaaagc aggtgaagaa gatccgagaa ccgtgtctca agtgtgtgga 2041 catggttatc tcggagctaa tcagcaccgt tagacagtgc accaagaagc tccagcagta 2101 cccgcggcta cgggaggaga tggagcgcat cgtgaccacc cacatccggg agcgcgaggg 2161 ccgcactaag gagcaggtca tgcttctcat cgatatcgag ctggcttaca tgaacaccaa 2221 ccatgaggac ttcataggct ttgccaatgc tcagcagagg agcaaccaga tgaacaagaa 2281 gaagacttca gggaaccagg atgagattct ggtcatccgc aagggctggc tgactatcaa 2341 taatattggc atcatgaaag ggggctccaa ggagtactgg tttgtgctga ctgctgagaa 2401 tctgtcctgg tacaaggatg atgaggagaa agagaagaaa tacatgctgt ctgtggacaa 2461 cctcaagctg cgggacgtgg agaagggctt tatgtcgagc aagcatatct ttgccctctt 2521 taacacggag cagaggaatg tctacaagga ttatcggcag ctggagctag cctgtgagac 2581 acaggaggag gtggacagct ggaaggcctc cttcctgagg gctggcgtgt accctgagcg 2641 tgttggggac aaagagaaag ccagcgagac cgaggagaat ggctccgaca gcttcatgca 2701 ttccatggac ccacagctgg aacggcaagt ggagaccatc cggaatcttg tggactcata 2761 catggccatt gtcaacaaga ccgtgaggga cctcatgccc aagaccatca tgcacctcat 2821 gattaacaat accaaggagt tcatcttctc ggagctgctg gccaacctgt actcgtgtgg 2881 ggaccagaac acgctgatgg aggagtcggc ggagcaggca cagcggcgcg acgagatgct 2941 gcgcatgtac cacgcactga aggaggcgct cagcatcatc ggcaacatca acacgaccac 3001 cgtcagcacg cccatgcccc cgcccgtgga cgactcctgg ctgcaggtgc agagcgtacc 3061 ggccggacgc aggtcgccca cgtccagccc cacgccgcag cgccgagccc ccgccgtgcc 3121 cccagcccgg cccgggtcgc ggggccctgc tcctgggcct ccgcctgctg ggtccgccct 3181 ggggggggcg ccccccgtgc cctccaggcc gggggcttcc cctgaccctt tcggccctcc 3241 ccctcaggtg ccctcgcgcc ccaaccgcgc cccgcccggg gtccccagcc gatcgggtca 3301 ggcaagtcca tcccgtcctg agagccccag gccccccttc gacatcgagc tcaagcttcg 3361 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggcctcctc 3421 cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg 3481 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 3541 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccctca 3601 gttccagtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3661 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3721 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct 3781 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga 3841 ggcctccacc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagatgag 3901 gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct acatggccaa 3961 gaagcccgtg cagctgcccg gcgcctacaa gaccgacatc aagctggaca tcacctccca 4021 caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg 4081 cgcctaaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta 4141 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt 4201 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 4261 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 4321 aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt 4381 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc 4441 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 4501 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 4561 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg 4621 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 4681 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 4741 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg 4801 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 4861 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 4921 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc 4981 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 5041 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 5101 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 5161 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 5221 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 5281 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 5341 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 5401 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 5461 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc 5521 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 5581 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 5641 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 5701 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 5761 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 5821 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca 5881 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 5941 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 6001 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 6061 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 6121 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 6181 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 6241 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact 6301 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag 6361 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg 6421 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt 6481 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg 6541 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat 6601 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 6661 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 6721 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 6781 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 6841 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 6901 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 6961 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 7021 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 7081 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 7141 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 7201 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 7261 gagcgtcgat ttttgtgatg ctcgtc //