LOCUS Exported 9560 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLenti X1 GFP-Zeo DEST (720-2) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9560) AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. TITLE A versatile viral system for expression and depletion of proteins in mammalian cells. JOURNAL PLoS One. 2009 Aug 6;4(8):e6529. PUBMED 19657394 REFERENCE 2 (bases 1 to 9560) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9560 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(350..369) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1652..1671 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1905..1922 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2039..2060 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2075..2105 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2118..2140 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2137..2153 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2137..2153 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2172..2192 /label=T3 /note="T3 promoter, forward primer" promoter 2174..2192 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" LTR 2448..2628 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2672..2797 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3290..3523 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3707..3751 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4012..4129 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 4167..4291 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 4316..4346 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 4400..5059 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(4466..4485) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 5401..5706 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 5585..5604 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(5741..5865) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" misc_feature 5896..6484 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5949..5969) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6367..6378) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" enhancer 6559..6862 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 6863..7066 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 7017..7037 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 7105..7114 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 7111..7827 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 7111..7827 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(7156..7177) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(7417..7436) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7764..7785 /label=EGFP-C /note="EGFP, forward primer" CDS 7840..8208 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF ALRDPAGNCVHFVAEEQD" LTR 8301..8534 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8606..8727 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8643..8662) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8697..8716 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 8746..8881 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8808..8827 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(8913..8932) /label=T7 /note="T7 promoter, forward primer" promoter complement(8914..8932) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8942..8959) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8942..8958) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8951..8973) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9103..9558 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9190..9209) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9400..9421 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta ttccagtgtg 4141 gtggaattct gcagctgcag atatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt 4201 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat 4261 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac 4321 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgg cgagattttc 4381 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc 4441 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa 4501 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa 4561 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg 4621 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt 4681 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg 4741 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt 4801 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac 4861 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg 4921 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc 4981 cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga 5041 gtggcagggc ggggcgtaaa gatctggatc cggcttacta aaagccagat aacagtatgc 5101 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat 5161 gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga cagttgacag cgacagctat 5221 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga 5281 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg 5341 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg gactggtgaa 5401 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta 5461 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt 5521 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc 5581 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg 5641 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga 5701 atataaatgt caggctccgt tatacacagc cagtgtcgac catagtgact ggatatgttg 5761 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt 5821 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttgata tccagcacag 5881 tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 5941 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 6001 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 6061 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 6121 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 6181 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 6241 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 6301 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 6361 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 6421 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 6481 ccgcctggaa ttctgcatta gttattaata gtaatcaatt acggggtcat tagttcatag 6541 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 6601 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 6661 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 6721 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 6781 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 6841 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 6901 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 6961 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 7021 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg 7081 tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc 7141 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 7201 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 7261 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 7321 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 7381 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 7441 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 7501 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 7561 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 7621 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 7681 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 7741 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 7801 actctcggca tggacgagct gtacaagtcc ggactcacca agttgaccag tgccgttccg 7861 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc 7921 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc 7981 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc 8041 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc 8101 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc 8161 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga 8221 gatttaaatg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 8281 tttaaaagaa aaggggggac tggaagggct agctcactcc caacgaagac aagatctgct 8341 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggctg 8401 cctagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 8461 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 8521 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 8581 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 8641 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 8701 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact 8761 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 8821 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 8881 ctaggctttt gcgtcgagac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct 8941 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 9001 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 9061 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg 9121 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 9181 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 9241 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 9301 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 9361 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 9421 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 9481 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 9541 tattaacgtt tacaatttcc //