LOCUS Exported 7077 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pJL43.1 - Pglh-2::MosTase::glh-2utr SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7077) AUTHORS Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, TITLE Single-copy insertion of transgenes in Caenorhabditis elegans PUBMED 18953339 REFERENCE 2 (bases 1 to 7077) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7077 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 24..128 /gene="bla" /label=AmpR promoter CDS 129..989 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(347..366) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1160..1748 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1649..1668 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1902..1919 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2036..2057 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2072..2102 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2110..2126 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2115..2137 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2134..2150 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2134..2150 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2169..2189 /label=T3 /note="T3 promoter, forward primer" promoter 2171..2189 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind 2218..2234 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 2219..2235 /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6385..6401) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6385..6401) /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(6433..6452) /label=T7 /note="T7 promoter, forward primer" promoter complement(6434..6452) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6462..6479) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6462..6478) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6471..6493) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 6620..7075 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6707..6726) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6917..6938 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg 2221 aggtcgacgg tatcgataag cttgatatcg aattcatatt accattattc ttgaactttt 2281 atagtctttg gtagtggttt cgtgtagaaa ggcattggag tcgaaaggtt tgaaagtggt 2341 tcagaagaat ccgagtcgaa atgaatccca gaatctacaa aaacttttat taaacaattt 2401 agagcttttg agagtgtcat gttaaatttg cattcataac tgttttgtta tctgtttcta 2461 ctaattagtt ccaccgaatc aggatgcagt gagcgaaacc gaaatcgtga atttcggttt 2521 cgcccacagg ggacgaaaag taggttacta atatgtgaag cttttaaaaa ctccataaat 2581 tttgtgaact ccacagctag gatcaagggt gccgatttat cgtttttcag gaaaacattt 2641 ttttgtttac atttttttaa aaagttgcgg gttttctcgt tttagaataa attcatgttt 2701 ccttaatttt tttttcatca acacaaaaaa ttgattatta aacattttct taaaaaattt 2761 tccgattttt cgtttttcag gaaaatcgaa attttttcgc ttttccgata tttcgaaaaa 2821 tatttcattt ttagagttta tcttcttttt agaagaaaat tatgttttct tatgattttt 2881 cccatcaaca aaacaataat tttttgaaac tgatttttct ttgttaactt tctaaatttt 2941 tttatttttc aggaaaatcg aacaatcgaa aattgttcat ttttcatgat accctaaaaa 3001 tataaagttt gatcgatata atctatttca atcttatgaa aatttttaaa ataaactgac 3061 cagaatcgga tgttgcttcg gtagctgaga ggttccattg ttcttcctca tcgacttcat 3121 catcactatc ttcttcaatc gttgttacta cccattgttt tccaaccaat ttttcattgc 3181 acttgaaatt ttcaagggct ttcatactac tttgatacat ttccaatgcc gaattatgct 3241 cgttagtcat caaatcctca gcaggagaaa caccatccga gaaggaatac tttccaaaac 3301 caacagagaa taaactttga ttttccatat caaatccaga agacttgtag tttgatattg 3361 gtctagaaat ctggctgcag cgagtttcag agttacagcg agcaaaatca tattgtatca 3421 gagccgtgaa gtacgtgaat ttcttgtatg aaaccagttt ttgaagctgt ttgagaatga 3481 aaatatgttc atcgcaaggt ttttcagcat actttgaagc gtcacgtggg ttcagaatga 3541 gttttccgtc cggtaagatc tcaatcaacg tttcagtgtt aagctctggt tgtgtgacat 3601 actgcaattt tttaattgaa ggcaaaattt gatgttcaaa caaatttacg ttgaaaatcg 3661 ccgtccgaat cgcgtatact tcgttaaaat gaacgttgaa tgaccgaagg gattgaagaa 3721 tacaagctaa aacgtcgttg gcagtattcg tgatatgaac ccttaagaat attgtttaaa 3781 tttggccaaa ttcagagtta ttaccattga atcagaagtt gaataatcca tgatttttct 3841 tccataggca acggttctgc gaccattgca atcattgatt ggcatgaaat gtgtgccata 3901 tcgtcgtcct tcattgtatg gcttctcagt gactcgataa tttttttcgc gaggggttcg 3961 attccatcct tgctttgata atactgagtt atgtggctga gaagaactcc tcctcctcgc 4021 ggacgaggca aatagtcttg cattgaaagt tcgttgagag catgaattgc gtccagtaca 4081 ctaatattca tttctgaaaa aattaattaa attgtaaatt aaatatgcca aaagataaac 4141 gaacgtttaa gtttaaaaag gtctccagat taattaggga aactattttc agaaagaaaa 4201 attctgaagg ttccacaaag tattactggt atggcaaaaa gtgcgatgca aggatactga 4261 aattgtattc agaaaatccc aaattgagtt tttaaaaggc tcatacgagc agcataggac 4321 aagggtctcg ccacgttcgt cctctacagt actcccgcgc ggcaaatttc aaatcttttg 4381 cgcgcgagtt tctgagtttc cattcattta aaattttttg gtttgcaaac gccaaacttg 4441 tactttttcc tggaagttcg aagacgcgtt atgtcgagtt tcgtgccgaa taaagagcaa 4501 acgcggacag tattaatttt ctgttttcat ttgaagaaaa cagctgcgga atcgcaccga 4561 atgcttgttg aagcctttgg cgaacaagta ccaactgtga aaacgtgtga acggtggttt 4621 caacgcttca aaagtggtga ttttgacgtc gacgacaaag agcacggaaa accgccaaaa 4681 aggtacgaag acgccgaact gcaagcatta ttggatgaag acgatgctca aacgtaagtt 4741 taaacatata tactaactaa cccatggatt atttaaattt tcaggcaaaa acaactcgca 4801 gagcagttgg aagtaagtca acaagcagtt tccaatcgct tgcgagagat gggaaagatt 4861 cagaaggtcg gtagatgggt gccacatgag ttgaacgaga ggcagatgga gaggcgcaaa 4921 aacacatgcg aaattttgct ttcacgatac aaaaggaagt cgtttttgca tcgtatcgtt 4981 actggagatg aaaaatggat cttttttgtt aatcctaaac gtaaaaagtc atacgttgat 5041 cctggacaac cggccacatc gactgctcga ccgaatcgct ttggcaagaa gacgatgctc 5101 tgtgtttggt gggatcagag cggtgtcatt tactatgagc tcttgaaacc cggcgaaacg 5161 gtgaatacgg cacgctacca acaacaattg atcaatttga accgtgcgct tcagagaaaa 5221 cgaccggaat atcaaaaaag acaacacagg gtcatttttc tccatgacaa cgctccatca 5281 catacggcaa gagcggttcg cgacacgttg gaaacactca attgggaagt gcttccgcat 5341 gcggcttact caccagacct ggccccatcc gattaccacc tattcgcttc gatgggacac 5401 gcactcgctg agcagcgctt cgattcttac gaaagtgtga aaaaatggct cgatgaatgg 5461 ttcgccgcaa aagacgatga gttctactgg cgtggaatcc acaaattgcc cgagagatgg 5521 gaaaaatgtg tcgcgagcga cggcaaatac tttgaatagc tagcatgtat ctgcatatcc 5581 ttcaaatgtt gtccatattg tatcagtaaa ttataatgcc ccctttttat atattctcct 5641 tatcatgtaa atgttctatt gattttttgc tggtgtaaaa cgtttttata gtaaatcctc 5701 tccacagtga atttgttaaa ttgtatcaaa acaagacatt gattaaattt gttcgagact 5761 tgtggattgt aaaatatttt gcagaaaatg gcaatattca tttagaaaca ttacttccat 5821 aataggaaaa actatttatg agttttcatg tgcctatgca agttcccact ctgtgtgaat 5881 ttcatcatac aaattgagca aacataaggt ttatgtccag tatgggttct cagatgtttt 5941 gtaagagttg agctgtcggt gaatgccttc tcacattgag cacatggata cggtctctct 6001 ccagtgtgtg ttctgaaata atgaaaaata gtcttgcaat atgtttctac tattcgagaa 6061 aaaatgtaat tttttttctt atggtaacct gcaatttata tattttttat ttgccgttat 6121 tgacattatt ataagaaatt ggaggatatc acaaatatcg taaattctcc aaaattttga 6181 cacttcactt taaagacaaa tgcggttttt ccgagtgtgt ctcgccacga tctcagtcat 6241 aatggtcgct acaggtgcca aactttaggc atttgttata tttgatgcag aaaaccaagc 6301 acatgaaaat taaaattgtc aagtagacaa tgaaatccag acattgttat gattcatttt 6361 tttgcgaatt cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg 6421 agctccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa 6481 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 6541 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 6601 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 6661 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 6721 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 6781 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 6841 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 6901 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 6961 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 7021 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttag //