LOCUS Exported 4809 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pJL44 - Phsp16.48::MosTase::glh-2utr SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4809) AUTHORS Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, TITLE Single-copy insertion of transgenes in Caenorhabditis elegans PUBMED 18953339 REFERENCE 2 (bases 1 to 4809) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4809 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 95..401 /label=hsp16-48 promoter /note="promoter for the C. elegans heat shock protein 16-48 gene (Russnak and Candido, 1985)" primer_bind complement(2383..2405) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 2505..2524 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(2602..2620) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2688..2792 /gene="bla" /label=AmpR promoter CDS 2793..3653 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3011..3030) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3824..4412 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4313..4332 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4566..4583 /label=L4440 /note="L4440 vector, forward primer" protein_bind 4700..4721 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 4736..4766 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4774..4790 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 4779..4801 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 4798..5 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 4798..5 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 atgaccatga ttacgccaag cttgctggac ggaaatagtg gtaaagtgac atgattgtag 61 tttgaagatt tcacaattag agtgaatgtt gtttggttcg gttttgtcac tgtatttata 121 ctcatttcca cctttttcta gaacattcga gctgcttctt gcaaaaggag ggcgactcac 181 attcagaaca ttgagaaata gtgtgcgtac tgaagaaacc cagatacttt ttcaatctgc 241 gtctctttgc acctatgggg tgtattttga aatgaatgca tctaggacct tctagaacat 301 tctaaacggc tgcaggatac gggtatataa gccaatcgtg ttcagaggaa accaatacac 361 tttgttcaag tgcttactgt tcattctcta aacttcaaga acgcgttatg tcgagtttcg 421 tgccgaataa agagcaaacg cggacagtat taattttctg ttttcatttg aagaaaacag 481 ctgcggaatc gcaccgaatg cttgttgaag cctttggcga acaagtacca actgtgaaaa 541 cgtgtgaacg gtggtttcaa cgcttcaaaa gtggtgattt tgacgtcgac gacaaagagc 601 acggaaaacc gccaaaaagg tacgaagacg ccgaactgca agcattattg gatgaagacg 661 atgctcaaac gtaagtttaa acatatatac taactaaccc atggattatt taaattttca 721 ggcaaaaaca actcgcagag cagttggaag taagtcaaca agcagtttcc aatcgcttgc 781 gagagatggg aaagattcag aaggtcggta gatgggtgcc acatgagttg aacgagaggc 841 agatggagag gcgcaaaaac acatgcgaaa ttttgctttc acgatacaaa aggaagtcgt 901 ttttgcatcg tatcgttact ggagatgaaa aatggatctt ttttgttaat cctaaacgta 961 aaaagtcata cgttgatcct ggacaaccgg ccacatcgac tgctcgaccg aatcgctttg 1021 gcaagaagac gatgctctgt gtttggtggg atcagagcgg tgtcatttac tatgagctct 1081 tgaaacccgg cgaaacggtg aatacggcac gctaccaaca acaattgatc aatttgaacc 1141 gtgcgcttca gagaaaacga ccggaatatc aaaaaagaca acacagggtc atttttctcc 1201 atgacaacgc tccatcacat acggcaagag cggttcgcga cacgttggaa acactcaatt 1261 gggaagtgct tccgcatgcg gcttactcac cagacctggc cccatccgat taccacctat 1321 tcgcttcgat gggacacgca ctcgctgagc agcgcttcga ttcttacgaa agtgtgaaaa 1381 aatggctcga tgaatggttc gccgcaaaag acgatgagtt ctactggcgt ggaatccaca 1441 aattgcccga gagatgggaa aaatgtgtcg cgagcgacgg caaatacttt gaatagctag 1501 catgtatctg catatccttc aaatgttgtc catattgtat cagtaaatta taatgccccc 1561 tttttatata ttctccttat catgtaaatg ttctattgat tttttgctgg tgtaaaacgt 1621 ttttatagta aatcctctcc acagtgaatt tgttaaattg tatcaaaaca agacattgat 1681 taaatttgtt cgagacttgt ggattgtaaa atattttgca gaaaatggca atattcattt 1741 agaaacatta cttccataat aggaaaaact atttatgagt tttcatgtgc ctatgcaagt 1801 tcccactctg tgtgaatttc atcatacaaa ttgagcaaac ataaggttta tgtccagtat 1861 gggttctcag atgttttgta agagttgagc tgtcggtgaa tgccttctca cattgagcac 1921 atggatacgg tctctctcca gtgtgtgttc tgaaataatg aaaaatagtc ttgcaatatg 1981 tttctactat tcgagaaaaa atgtaatttt ttttcttatg gtaacctgca atttatatat 2041 tttttatttg ccgttattga cattattata agaaattgga ggatatcaca aatatcgtaa 2101 attctccaaa attttgacac ttcactttaa agacaaatgc ggtttttccg agtgtgtctc 2161 gccacgatct cagtcataat ggtcgctaca ggtgccaaac tttaggcatt tgttatattt 2221 gatgcagaaa accaagcaca tgaaaattaa aattgtcaag tagacaatga aatccagaca 2281 ttgttatgat tcattttttt gcgaattcct gcagcccggg ggatccacta gtcggccgta 2341 cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 2401 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 2461 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 2521 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 2581 ccgcatcagg cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 2641 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 2701 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 2761 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 2821 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 2881 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 2941 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 3001 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 3061 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 3121 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 3181 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 3241 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 3301 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 3361 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 3421 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 3481 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 3541 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 3601 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 3661 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 3721 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 3781 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 3841 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 3901 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 3961 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 4021 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 4081 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 4141 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 4201 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 4261 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 4321 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 4381 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 4441 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 4501 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 4561 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 4621 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 4681 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 4741 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 4801 gaaacagct //