LOCUS Exported 875 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.. ACCESSION . VERSION . KEYWORDS CMVp-dCas9-3xNLS-VP64 (Construct 1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 875) AUTHORS Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK TITLE Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. JOURNAL Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022. PUBMED 24837679 REFERENCE 2 (bases 1 to 875) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..875 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(46..195) /codon_start=1 /product="tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)" /label=VP64 /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF DLDML" CDS complement(235..255) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(262..282) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(289..309) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 ttgtaatcca gaggttgatt atcgataagc ttgatatcgt catgagagca tatccaggtc 61 aaaatcatcc aaggcatctg acccgagcat gtcgaggtcg aaatcgtcga gagcgtcgct 121 gccgagcata tccagatcga aatcatccag ggcgtcagaa ccgagcatat cgagatcgaa 181 atcgtccaaa gcgtctgaac ctccaccgcc gttgctgcct gagccgatcc catccacctt 241 cctctttttc ttggggtctt caaccttcct tttcttcttt ggatcctcga ctttgcgttt 301 cttcttgggt gaccctgaac ccagtgaggg cccgtcagcc ctgctgtctc caccgagctg 361 agagaggtcg attcttgttt catagagccc cgtaattgac tgatgaatca gtgtggcgtc 421 caggacctcc tttgtagagg tgtaccgctt tctgtctatg gtggtgtcga agtacttgaa 481 ggctgcaggc gcgcccaagt tggtcagagt aaacaagtgg ataatgtttt ctgcctgctc 541 cctgatgggc ttatccctgt gcttattgta agcagaaagc accttatcga ggttagcgtc 601 ggcgaggatc actcttttgg agaattcgct tatttgctcg atgatctcat caaggtagtg 661 tttgtgttgt tccacgaaca gctgcttctg ctcattatct tcgggagacc ctttgagctt 721 ttcatagtgg ctggccagat acaagaaatt aacgtattta gagggcagtg ccagctcgtt 781 acctttctgc agctcgcccg cactagcgag cattcgtttc cggccgtttt caagctcaaa 841 gagagagtac ttgggaagct taatgatgag gtctt //