LOCUS Exported 820 bp ds-DNA linear SYN 12-JUN-2021 DEFINITION Encodes module 2 with amino acids 12N-16Q for recognition of nt 7U. ACCESSION . VERSION . KEYWORDS pChlr-2NQ SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 820) AUTHORS Abil Z, Denard CA, Zhao H TITLE Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. JOURNAL J Biol Eng. 2014 Mar 1;8(1):7. PUBMED 24581042 REFERENCE 2 (bases 1 to 820) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..820 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(153..169) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(153..169) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(166..188) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 177..193 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(201..231) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 246..267 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(384..401) /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(635..654) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgagctcggt ctcaccaggc tgcctaccaa ctcatggtgg atgtgtttgg taattacgtc 61 attcagaagt tctttgaatt tggcagtctt gaacagaagc tggctttggc agaacggatt 121 cgaggtagag acccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 181 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 241 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 301 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 361 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 421 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 481 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 541 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 601 tcaagtcaga ggtggtgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 661 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 721 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 781 taggtcgttc gctccaagct gggctgtgtg cacgaacccc //