LOCUS Exported 823 bp ds-DNA linear SYN 07-JUN-2021 DEFINITION Encodes module 4 with amino acids 12N-13Y-16Q for recognition of nt 5U. ACCESSION . VERSION . KEYWORDS pChlr-4NYQ SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 823) AUTHORS Abil Z, Denard CA, Zhao H TITLE Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. JOURNAL J Biol Eng. 2014 Mar 1;8(1):7. PUBMED 24581042 REFERENCE 2 (bases 1 to 823) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 7, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..823 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(157..173) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(157..173) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(170..192) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 181..197 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(205..235) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 250..271 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(388..405) /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(639..658) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcgagctcgg tctcatggcc atgtcttgaa gtgtgtgaaa gatcagaatg gcaattacgt 61 ggttcagaaa tgcattgaat gtgtacagcc ccagtctttg caatttatca tcgatgcgtt 121 taagggacag gagagaccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 181 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 241 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 301 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 361 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 421 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 481 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 541 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 601 acgctcaagt cagaggtggt gaaacccgac aggactataa agataccagg cgtttccccc 661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 721 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccc //