LOCUS Exported 861 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Localization: C1 Cloning Vector, Excitation: 400 / 485, Emission: 468 / 511. ACCESSION . VERSION . KEYWORDS PA-sfGFP-C1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 861) TITLE Michael Davidson Empty Backbones REFERENCE 2 (bases 1 to 861) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..861 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 33..42 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 39..755 /codon_start=1 /product="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" /label=superfolder GFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSVLSKDPNEKRDHMVLL EFVTAAGITHGMDELYK" primer_bind complement(84..105) /label=EGFP-N /note="EGFP, reverse primer" primer_bind 692..713 /label=EGFP-C /note="EGFP, forward primer" misc_feature 756..821 /label=MCS /note="multiple cloning site" ORIGIN 1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc 61 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 121 tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca 181 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgagctacg 241 gcgtgcagtg cttcagccgc taccccgacc acatgaagcg ccacgacttc ttcaagtccg 301 ccatgcccga aggctacgtc caggagcgca ccatcagctt caaggacgac ggcacctaca 361 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 421 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca 481 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga 541 tccgccacaa cgtggaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 601 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagccac cagtccgtgc 661 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 721 ccgggatcac tcacggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag 781 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga 841 tcataatcag ccataccaca t //