LOCUS Exported 1058 bp ds-DNA linear SYN 11-JUN-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pET His6 High pI protein 69 TEV LIC cloning vector (2-HpI-69) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1058) TITLE 2-series LIC N-terminal fusion vectors for E. coli expression REFERENCE 2 (bases 1 to 1058) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 11, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1058 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 201..220 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind 304..323 /label=T7 /note="T7 promoter, forward primer" promoter 304..322 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 411..433 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 453..470 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 972..983 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" CDS 1029..1049 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" ORIGIN 1 ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 61 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 121 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 181 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 241 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 301 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag 361 agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat tttgtttaac 421 tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat ggttcttcta 481 tgaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc gaaattcgcg 541 cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg agtctgagag 601 aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc cctaacgccg 661 agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag agcaagtctt 721 ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa ttccgtcctg 781 gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt ctcgaagagg 841 gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac cagcaaatcg 901 gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagtg gtcgaatcct 961 tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag aagaaacagg 1021 ggatcgagga aaacctgtac ttccaatcca atattgga //