LOCUS Exported 1029 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG. . ACCESSION . VERSION . KEYWORDS pEVOL-pBpF SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1029) AUTHORS Chin JW, Martin AB, King DS, Wang L, Schultz PG TITLE Addition of a photocrosslinking amino acid to the genetic code of Escherichiacoli. JOURNAL Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11020-4. Epub 2002 Aug 1. PUBMED 12154230 REFERENCE 2 (bases 1 to 1029) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1029 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 156..173 /label=pBAD Reverse /note="For vectors with E. coli araBAD promoter, reverse primer" CDS complement(229..246) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" ORIGIN 1 ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac 61 ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag 121 gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa 181 tcttctctca tccgccaaaa cagccaagct ggagaccgtt taaactcaat gatgatgatg 241 atgatggtcg acttattata atctctttct aattggctct aaaatcttta taagttcttc 301 agctacagca ttttttaaat ccattggatg caattcctta tttttaaata aactctctaa 361 ctcctcatag ctattaactg tcaaatctcc accaaatttt tctggccttt ttatggttaa 421 aggatattca aggaagtatt tagctatctc cattattgga tttccttcaa caactccagc 481 tgggcagtat gctttcttta tcttagccct aatctcttct ggagagtcat caacagctat 541 aaaattccct tttgaagaac tcatctttcc ttctccatcc aaacccgtta agacagggtt 601 gtgaatacaa acaacctttt ttggtaaaag ctcccttgct aacatgtgta tttttctctg 661 ctccatccct ccaactgcaa catcaacgcc cagataatga ctcgtattaa cctgcattat 721 tggatagata acttcagcaa cctttggatt ttcatcctct cttgctataa gttccatact 781 ccttcttgct ctttttaagg tagtttttaa agccaatcta tagacattca gtgtataatc 841 cttatcaagc tggaaaggac ttccataaag atattttgcc tttaacccca ttgcttcaaa 901 aacttttttg ttataatctc ctatttttct aatctcatcc aactctcctt tctggtttaa 961 ataggcgtgt aaatcagcca acaatataat tatatcaaat ccagcatttt gtaaatcaat 1021 catcttttt //