LOCUS       Exported                1149 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  tRNA synthetase/tRNA pair for the in vivo incorporation of a 
            photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli 
            in response to the amber codon, TAG. .
ACCESSION   .
VERSION     .
KEYWORDS    pEVOL-pBpF
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Chin JW, Martin AB, King DS, Wang L, Schultz PG
  TITLE     Addition of a photocrosslinking amino acid to the genetic code of 
            Escherichiacoli.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11020-4. Epub 2002 Aug 
            1.
  PUBMED    12154230
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(44..922)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPSDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gcttggtacc caattatgac aacttgacgg
       61 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
      121 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
      181 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
      241 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
      301 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
      361 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
      421 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
      481 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
      541 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
      601 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
      661 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcactcg
      721 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
      781 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
      841 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
      901 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
      961 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca
     1021 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     1081 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     1141 gcgtcacac
//
