LOCUS Exported 10712 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage. ACCESSION . VERSION . KEYWORDS VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10712) AUTHORS Venkatachalam V, Brinks D, Maclaurin D, Hochbaum D, Kralj J, Cohen AE TITLE Flash memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin. JOURNAL J Am Chem Soc. 2014 Feb 12;136(6):2529-37. doi: 10.1021/ja411338t. Epub 2014 Jan 27. PUBMED 24428326 REFERENCE 2 (bases 1 to 10712) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10712 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2622..3833 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3670..3689 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" regulatory 3868..3877 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3874..4647 /codon_start=1 /product="light-driven proton pump archaerhodopsin-3 from Halorubrum sodomense (Chow et al., 2010)" /label=Arch /translation="MDPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT DKDAREYYAVTILVPGIASAAYLSMFFGIGLTEVTVGGEMLDIYYARYAQWLFTTPLLL LDLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYFLA TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD VTAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD" CDS 4663..5376 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(4705..4726) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4966..4985) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5313..5334 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5404..5992 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5457..5477) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5875..5886) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5995..6011) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5996..6012) /label=pBluescriptKS /note="For pBluescript vector" LTR 6517..6697 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6723..6740) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6729..6953 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6999..7427 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7086..7105) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7296..7317 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7436..7456) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7441..7770 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7621..7756 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7683..7702 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7818..7865 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7884..8258 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8388..8509 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8425..8444) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8479..8498 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8558..8574) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8558..8574) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8571..8593) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8582..8598 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8606..8636) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8651..8672 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8789..8806) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8960..9548) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9040..9059) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9719..10579) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10342..10361 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10580..10684) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt 2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg 2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga 2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc 2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg 2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc 2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga 3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc 3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt 3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt 3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg 3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc 3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg 3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc 3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt 3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc 3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct 3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc 3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg 3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt 3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt 3841 gggctgcagg tcgactctag aggatccgcc accatggacc ccatcgctct gcaggctggt 3901 tacgacctgc tgggtgacgg cagacctgaa actctgtggc tgggcatcgg cactctgctg 3961 atgctgattg gaaccttcta ctttctggtc cgcggatggg gagtcaccga taaggatgcc 4021 cgggaatatt acgctgtgac tatcctggtg cccggaatcg catccgccgc atatctgtct 4081 atgttctttg gtatcgggct tactgaggtg accgtcgggg gcgaaatgtt ggatatctat 4141 tatgccaggt acgcccagtg gctgtttacc accccacttc tgctgctgga tctggccctt 4201 ctcgctaagg tggatcgggt gaccatcggc accctggtgg gtgtggacgc cctgatgatc 4261 gtcactggcc tcatcggagc cttgagccac acggccatag ccagatacag ttggtggttg 4321 ttctctacaa tttgcatgat agtggtgctc tattttctgg ctacatccct gcgatctgct 4381 gcaaaggagc ggggccccga ggtggcatct acctttaaca ccctgacagc tctggtcttg 4441 gtgctgtgga ccgcttaccc tatcctgtgg atcataggca ctgagggcgc tggcgtggtg 4501 ggcctgggca tcgaaactct gctgtttatg gtgttggacg tgactgccaa ggtcggcttt 4561 ggctttatcc tgttgagatc ccgggctatt ctgggcgaca ccgaggcacc agaacccagt 4621 gccggtgccg atgtcagtgc cgccgaccga ccggtagtag cagtgagcaa gggcgaggag 4681 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 4741 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 4801 atttgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac 4861 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 4921 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 4981 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 5041 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 5101 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 5161 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 5221 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc 5281 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 5341 gccgggatca ctctcggcat ggacgagctg tacaagtaag aattcgatat caagcttatc 5401 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 5461 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 5521 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 5581 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 5641 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 5701 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 5761 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg 5821 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 5881 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 5941 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac 6001 cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 6061 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 6121 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 6181 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 6241 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca 6301 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag 6361 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat 6421 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt 6481 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc 6541 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 6601 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 6661 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc 6721 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 6781 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 6841 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 6901 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 6961 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 7021 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 7081 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 7141 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 7201 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 7261 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 7321 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 7381 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 7441 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 7501 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 7561 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 7621 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 7681 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 7741 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 7801 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat 7861 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg 7921 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact 7981 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg 8041 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg 8101 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg 8161 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg 8221 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt 8281 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 8341 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg 8401 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 8461 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta 8521 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 8581 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 8641 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 8701 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 8761 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 8821 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 8881 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 8941 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 9001 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 9061 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 9121 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 9181 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 9241 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 9301 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 9361 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 9421 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 9481 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 9541 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 9601 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 9661 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 9721 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 9781 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 9841 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 9901 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 9961 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 10021 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 10081 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 10141 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 10201 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 10261 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 10321 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 10381 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 10441 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 10501 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 10561 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 10621 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 10681 cgcgcacatt tccccgaaaa gtgccacctg ac //