LOCUS Exported 10618 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses GCaMP6f under the a-CamKII promoter. ACCESSION . VERSION . KEYWORDS VV229: GCaMP6f in fck SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10618) AUTHORS Venkatachalam V, Cohen AE TITLE Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. JOURNAL Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. PUBMED 25296307 REFERENCE 2 (bases 1 to 10618) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10618 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2602..3890 /label=CaMKII promoter /note="promoter for the mouse calcium/calmodulin-dependent kinase II alpha subunit" regulatory 3900..3909 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3906..5255 /codon_start=1 /product="improved fluorescent protein-based calcium sensor (Chen et al., 2013)" /label=GCaMP6f /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK" regulatory 4002..4011 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind 4292..4313 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(4419..4440) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4680..4699) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" misc_feature 5310..5898 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5363..5383) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5781..5792) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5901..5917) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5902..5918) /label=pBluescriptKS /note="For pBluescript vector" LTR 6423..6603 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6629..6646) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6635..6859 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6905..7333 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6992..7011) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7202..7223 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7342..7362) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7347..7676 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7527..7662 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7589..7608 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7724..7771 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7790..8164 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8294..8415 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8331..8350) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8385..8404 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8464..8480) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8464..8480) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8477..8499) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8488..8504 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8512..8542) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8557..8578 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8695..8712) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8866..9454) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8946..8965) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9625..10485) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10248..10267 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10486..10590) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt 2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag 2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat 2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca 2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg 2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga 2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg 3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga 3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac 3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg 3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa 3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt 3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga 3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc 3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa 3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag 3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac 3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag 3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac 3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg 3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt 3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccg 3901 ccaccatggg ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc 3961 aaatgggtcg ggatctgtac gacgatgacg ataaggatct cgccaccatg gtcgactcat 4021 cacgtcgtaa gtggaataag acaggtcacg cagtcagagc tataggtcgg ctgagctcac 4081 tcgagaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttcaaga 4141 tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc 4201 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccaaac 4261 tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 4321 ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga 4381 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 4441 taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc tacggcaagc 4501 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 4561 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg 4621 acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc ttcttcaagg 4681 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 4741 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 4801 agtacaacct gccggaccaa ctgactgaag agcagatcgc agaatttaaa gaggaattct 4861 ccctatttga caaggacggg gatgggacaa taacaaccaa ggagctgggg acggtgatgc 4921 ggtctctggg gcagaacccc acagaagcag agctgcagga catgatcaat gaagtagatg 4981 ccgacggtga cggcacaatc gacttccctg agttcctgac aatgatggca agaaaaatga 5041 aatacaggga cacggaagaa gaaattagag aagcgttcgg tgtgtttgat aaggatggca 5101 atggctacat cagtgcagca gagcttcgcc acgtgatgac aaaccttgga gagaagttaa 5161 cagatgaaga ggttgatgaa atgatcaggg aagcagacat cgatggggat ggtcaggtaa 5221 actacgaaga gtttgtacaa atgatgacag cgaagtacaa gttctgctac gagaacgagg 5281 tgtaagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa 5341 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 5401 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 5461 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 5521 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 5581 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 5641 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 5701 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 5761 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 5821 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 5881 tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa 5941 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg 6001 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg 6061 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 6121 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg 6181 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct 6241 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc 6301 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt 6361 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta 6421 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 6481 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 6541 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 6601 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 6661 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 6721 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 6781 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 6841 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat 6901 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 6961 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 7021 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 7081 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 7141 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 7201 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 7261 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 7321 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 7381 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 7441 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 7501 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 7561 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct 7621 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 7681 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc 7741 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt 7801 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac 7861 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga 7921 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc 7981 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac 8041 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg 8101 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga 8161 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 8221 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 8281 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 8341 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 8401 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc 8461 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 8521 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 8581 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 8641 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 8701 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 8761 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 8821 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 8881 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 8941 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 9001 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 9061 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 9121 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 9181 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 9241 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 9301 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 9361 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 9421 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 9481 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 9541 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 9601 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 9661 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 9721 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 9781 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 9841 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 9901 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 9961 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 10021 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 10081 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 10141 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 10201 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 10261 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 10321 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 10381 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 10441 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 10501 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 10561 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgac //