LOCUS Exported 1084 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION E coli genetic modification. ACCESSION . VERSION . KEYWORDS pMDIAI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1084) AUTHORS Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu J, Jiang W, Yang S TITLE High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. JOURNAL Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18. PUBMED 24747889 REFERENCE 2 (bases 1 to 1084) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1084 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(58..104) /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS complement(114..914) /codon_start=1 /gene="aac(3)IV" /product="aminoglycoside 3-N-acetyltransferase type IV " /label=ApmR /note="confers resistance to apramycin (Paget and Davies, 1996)" /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG" ORIGIN 1 acggcaagtg gacgattatt agggataaca gggtaatgtg taggctggag ctgcttcgaa 61 gttcctatac tttctagaga ataggaactt cggaatagga acttatgagc tcagccaatc 121 gactggcgag cggcatcgca ttcttcgcat cccgcctctg gcggatgcag gaagatcaac 181 ggatctcggc ccagttgacc cagggctgtc gccacaatgt cgcgggagcg gatcaaccga 241 gcaaaggcat gaccgactgg accttccttc tgaaggctct tctccttgag ccacctgtcc 301 gccaaggcaa agcgctcaca gcagtggtca ttctcgagat aatcgacgcg taccaacttg 361 ccatcctgaa gaatggtgca gtgtctcggc accccatagg gaacctttgc catcaactcg 421 gcaagatgca gcgtcgtgtt ggcatcgtgt cccacgccga ggagaagtac ctgcccatcg 481 agttcatgga cacgggcgac cgggcttgca ggcgagtgag gtggcagggg caatggatca 541 gagatgatct gctctgcctg tggccccgct gccgcaaagg caaatggatg ggcgctgcgc 601 tttacatttg gcaggcgcca gaatgtgtca gagacaactc caaggtccgg tgtaacgggc 661 gacgtggcag gatcgaacgg ctcgtcgtcc agacctgacc acgagggcat gacgagcgtc 721 cctcccggac ccagcgcagc acgcagggcc tcgatcagtc caagtggccc atcttcgagg 781 ggccggacgc tacggaagga gctgtggacc agcagcacac cgccgggggt aaccccaagg 841 ttgagaagct gaccgatgag ctcggctttt cgccattcgt attgcacgac attgcactcc 901 accgctgatg acatcagtcg atcatagcac gatcaacggc actgttgcaa atagtcggtg 961 gtgataaact tatcatcccc ttttgcttat ggagctgcac atgaacccat tcaaaggccg 1021 gcattttcag cgtgacatca ttctgtgggc cgtacgctgg tactgcaaat acggcatcag 1081 ttac //