LOCUS Exported 1100 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION E coli genetic modification. ACCESSION . VERSION . KEYWORDS pMDIAI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1100) AUTHORS Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu J, Jiang W, Yang S TITLE High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. JOURNAL Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18. PUBMED 24747889 REFERENCE 2 (bases 1 to 1100) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1100 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 91..124 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" oriT 131..240 /note="incP origin of transfer" ORIGIN 1 attacgaact cgtcctcggt tcccggcgat cctctggaga ttatattacc ctgttatccc 61 taattccggg gatccgtcga cctgcagttc gaagttccta ttctctagaa agtataggaa 121 cttcgaagtt cccgccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa 181 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc 241 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta 301 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt 361 tatgcagcgg aaaatgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg 421 cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca 481 taagcaaaag gggatgataa gtttatcacc accgactatt tgcaacagtg ccgttgatcg 541 tgctatgatc gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga 601 aaagccgagc tcatcggtca gcttctcaac cttggggtta cccccggcgg tgtgctgctg 661 gtccacagct ccttccgtag cgtccggccc ctcgaagatg ggccacttgg actgatcgag 721 gccctgcgtg ctgcgctggg tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg 781 gacgacgagc cgttcgatcc tgccacgtcg cccgttacac cggaccttgg agttgtctct 841 gacacattct ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc ctttgcggca 901 gcggggccac aggcagagca gatcatctct gatccattgc ccctgccacc tcactcgcct 961 gcaagcccgg tcgcccgtgt ccatgaactc gatgggcagg tacttctcct cggcgtggga 1021 cacgatgcca acacgacgct gcatcttgcc gagttgatgg caaaggttcc ctatggggtg 1081 ccgagacact gcaccattct //