LOCUS Exported 11020 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter. ACCESSION . VERSION . KEYWORDS VV221: sdChR(C138S)-TS-eGFP-ER in fck SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11020) AUTHORS Venkatachalam V, Cohen AE TITLE Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. JOURNAL Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. PUBMED 25296307 REFERENCE 2 (bases 1 to 11020) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11020 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2602..3890 /label=CaMKII promoter /note="promoter for the mouse calcium/calmodulin-dependent kinase II alpha subunit" regulatory 3900..3909 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3906..4853 /codon_start=1 /product="blue light-activated optogenetic actuator derived from Scherffelia dubia channelrhodopsin (Hochbaum et al., 2014)" /label=CheRiff /translation="MGGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQ CSSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKV ILEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTSPVILIHLSNITGLSEEYNKRTMALL VSDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLV LAMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKI HEHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV" CDS 4950..5663 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(4992..5013) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5253..5272) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5600..5621 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5712..6300 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5765..5785) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6183..6194) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6303..6319) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6304..6320) /label=pBluescriptKS /note="For pBluescript vector" LTR 6825..7005 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(7031..7048) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7037..7261 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7307..7735 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7394..7413) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7604..7625 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7744..7764) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7749..8078 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7929..8064 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7991..8010 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8126..8173 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8192..8566 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8696..8817 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8733..8752) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8787..8806 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8866..8882) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8866..8882) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8879..8901) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8890..8906 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8914..8944) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8959..8980 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9097..9114) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9268..9856) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9348..9367) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10027..10887) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10650..10669 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10888..10992) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt 2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag 2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat 2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca 2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg 2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga 2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg 3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga 3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac 3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg 3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa 3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt 3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga 3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc 3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa 3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag 3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac 3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag 3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac 3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg 3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccat 3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccg 3901 ccaccatggg cggagctcct gctccagacg ctcacagcgc cccacctgga aacgattctg 3961 ccggaggcag tgagtaccat gccccagctg gatatcaagt gaatccaccc taccaccccg 4021 tgcatgggta tgaggaacag tgcagctcca tctacatcta ctatggggcc ctgtgggagc 4081 aggaaacagc taggggcttc cagtggtttg ccgtgttcct gtctgccctg tttctggctt 4141 tctacggctg gcacgcctat aaggccagcg tgggatggga ggaagtgtac gtgtgctccg 4201 tggagctgat caaagtgatt ctggagatct atttcgagtt caccagtcct gctatgctgt 4261 tcctgtacgg agggaacatt accccatggc tgagatatgc cgaatggctg ctgacatctc 4321 ccgtgatcct gattcatctg tctaacatca ccggcctgag tgaggagtac aataagcgga 4381 caatggctct gctggtgtcc gacctgggaa ctatttgcat gggagtgaca gccgctctgg 4441 ccactgggtg ggtgaagtgg ctgttttact gtatcggcct ggtgtatgga acccagacat 4501 tctacaacgc tggaatcatc tacgtggagt cttactatat catgcctgcc ggcggctgta 4561 agaaactggt gctggccatg actgccgtgt actattctag ttggctgatg tttcccggcc 4621 tgttcatctt tgggcctgaa ggcatgcaca ccctgagcgt ggctgggtcc actattggcc 4681 ataccatcgc cgacctgctg tccaagaata tttggggact gctggggcac ttcctgcgga 4741 tcaaaattca cgagcatatc attatgtacg gcgatatcag gagaccagtg agctcccagt 4801 ttctgggacg caaggtggac gtgctggcct tcgtgacaga ggaagataaa gtggcggcac 4861 cggtagtagc agtgagcaag gcggccgcca agagcaggat caccagcgag ggcgagtaca 4921 tccccctgga ccagatcgac atcaacgtgg tgagcaaggg cgaggagctg ttcaccgggg 4981 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 5041 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatt tgcaccaccg 5101 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 5161 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 5221 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 5281 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 5341 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 5401 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 5461 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 5521 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 5581 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 5641 tcggcatgga cgagctgtac aaattctgct acgagaatga agtgtaagaa ttcgatatca 5701 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 5761 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 5821 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 5881 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 5941 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 6001 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 6061 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 6121 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 6181 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 6241 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 6301 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag 6361 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc 6421 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 6481 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata 6541 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac 6601 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg 6661 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga 6721 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc 6781 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag 6841 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 6901 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 6961 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc 7021 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 7081 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 7141 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 7201 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 7261 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc 7321 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 7381 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 7441 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 7501 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 7561 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 7621 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 7681 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc 7741 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 7801 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 7861 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 7921 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 7981 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 8041 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 8101 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat 8161 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt 8221 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc 8281 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat 8341 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg 8401 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc 8461 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga 8521 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga 8581 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 8641 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 8701 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 8761 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 8821 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 8881 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 8941 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 9001 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 9061 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 9121 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 9181 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 9241 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 9301 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 9361 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 9421 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 9481 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 9541 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 9601 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 9661 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 9721 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 9781 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 9841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 9901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 9961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 10021 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 10081 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 10141 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 10201 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 10261 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 10321 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 10381 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 10441 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 10501 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 10561 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 10621 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 10681 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 10741 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 10801 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 10861 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 10921 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 10981 aggggttccg cgcacatttc cccgaaaagt gccacctgac //