LOCUS Exported 4138 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCM3.19 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4138) TITLE Fourth generation Seydoux lab vectors REFERENCE 2 (bases 1 to 4138) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4138 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 73..100 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 192..278 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 331..430 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" terminator 1990..2313 /gene="tbb-2" /label=tbb-2 terminator /note="transcription terminator for the C. elegans tubulin beta-2 gene" protein_bind complement(2314..2413) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2525..2544) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2536..3345 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2603..2622) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3488..4076 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3977..3996 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt 61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca 121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc 181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga 241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct 301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg 361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa 421 aaagcaggct ccatgagaga gatcgtccac gtgcaagccg gacaatgcgg aaaccaaatc 481 ggatccaaat tctgggaggt catctccgac gagcacggaa tccagccaga cggaaccttc 541 aagggagaga cggatctcca gcttgagcgc attgacgtct actacaacga agccaacagt 601 gagtttttaa aattttctgg agatttcgaa atcgtttagt tggaatgatt aattaattaa 661 attttttcag acggaaagta tgtgccacgc gccgtgttgg tcgatctcga accaggaacc 721 atggattctg tccgctctgg accattcggc cagctgttcc gtcctgacaa ctttgtgttc 781 ggacaaagcg gagccggaaa caactgggcc aagggtcact acacagaagg agctgagctt 841 gtcgacaatg tgcttgacgt gatccgcaag gaagccgaag gatgcgattg tcttcaagga 901 ttccaactca ctcactctct cggaggagga accggatctg gaatgggaac acttctcatc 961 tccaaaatcc gcgaagagta cccagacaga atcatgagct cattctcggt tgtaccatcg 1021 ccaaaggtgt ccgacacagt tgtagaacca tacaatgcca ccctctccgt ccaccagctt 1081 gttgagaaca ccgatgagac ctactgcatt gacaacgagg ccctctacga catctgctac 1141 cgtaccctca aactcaccaa cccaacctac ggtgatttga accatcttgg taagttatta 1201 tttcttgttc aagtttaagt tatgcacaag ttacccaaat tgaaacctaa taattacaat 1261 tttcagtatc cctcaccatg tccggagtta ccacctgtct ccgattccca ggacagctca 1321 atgccgatct ccgcaagctt gccgtcaaca tggttccatt cccacgtctt cacttcttca 1381 tgccaggatt cgcaccactt tccgccaagg gaacccaggc ttaccgtgcc cttaccgtcg 1441 ccgagctcac ccaacaaatg ttcgatgcca agaacatgat ggctgcctgt gacccaagac 1501 acggacgtta cctgaccgtg gccgctatgt tccgtggacg catgtcgatg agagaggttg 1561 acgagcaaat gctcaacgtt cagaacaaga actcgtccta cttcgtcgaa tggatcccga 1621 acaacgtcaa gaccgccgtt tgtgacatcc caccaagagg actcaagatg gctgccacct 1681 tcgttggaaa ctcgaccgct atccaagagc ttttcaagcg tatctcagag cagttcactg 1741 gtaagaaatt tgaaactatc atccagtatc agttttaaaa acgttatttt ttcagctatg 1801 ttccgccgca aggctttcct ccattggtac actggagaag gaatggacga gatggagttc 1861 accgaagccg agagcaacat gaacgatctc atctcagaat accaacaata ccaagaagca 1921 actgccgaag acgacgtcga cggatacgct gagggagaag ctggagagac ttacgaatcg 1981 gagcaataaa tgcaagatcc tttcaagcat tcccttcttc tctatcactc ttctttcttt 2041 ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt ttatgacttt 2101 ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat gtgattctgt 2161 ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa cccgaaaggt 2221 tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc caacaaaaag 2281 aggaaacttt attgtgccgc caagaaaaaa gtcacccagc tttcttgtac aaagtgggca 2341 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata 2401 aaatcattat ttgccatcca gctgcagctc tggcccgtgt ctcaaaatct ctgatgttac 2461 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt 2521 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc 2581 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt 2641 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc 2701 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa 2761 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc 2821 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt 2881 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt 2941 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat 3001 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa 3061 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt 3121 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt 3181 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt 3241 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat 3301 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat 3361 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca 3421 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 3481 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 3541 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 3601 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 3661 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 3721 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 3781 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 3841 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 3901 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 3961 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 4021 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 4081 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt //