LOCUS Exported 985 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein). ACCESSION . VERSION . KEYWORDS pRSETa mEos2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 985) AUTHORS McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL TITLE A bright and photostable photoconvertible fluorescent protein. JOURNAL Nat Methods. 2009 Feb . 6(2):131-3. PUBMED 19169260 REFERENCE 2 (bases 1 to 985) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..985 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 3..25 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 45..62 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 66..98 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 102..125 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" CDS 141..821 /codon_start=1 /product="monomeric variant of green-to-red photoswitchable fluorescent protein EosFP (McKinney et al., 2009)" /label=mEos2 /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD NARR" primer_bind complement(884..902) /label=T7 Term /note="T7 terminator, reverse primer" terminator 898..945 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" ORIGIN 1 attttgttta actttaagaa ggagatatac atatgcgggg ttctcatcat catcatcatc 61 atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg 121 ataaggatcg atggggatcc atgagtgcga ttaagccaga catgaagatc aaactccgta 181 tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt 241 ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct 301 ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccaaa tatccagaca 361 acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga 421 ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa ggggacactt 481 tctataataa agttcgattt tatggtacca actttcccgc caatggtcca gttatgcaga 541 agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga 601 cgggtgatat tcatatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca 661 gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac tttgtggacc 721 actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg 781 ctgttgctca ttctggattg cctgacaatg ccagacgata agaattcgaa gcttgatccg 841 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta 901 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact 961 atatccggat ctggcgtaat agcga //