LOCUS       Exported                1035 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  bacterial expression of an EosFP variant, mEos2 (a photoconvertible 
            fluorescent protein).
ACCESSION   .
VERSION     .
KEYWORDS    pRSETa mEos2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(39..719)
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             complement(735..758)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(762..794)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(798..815)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             835..857
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(888..907)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(889..907)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(992..1009)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcaagct tcgaattctt atcgtctggc attgtcaggc
       61 aatccagaat gagcaacagc atgctcatac agcttaacct tgttgtaatc tttgtcatgg
      121 cttaaaatct caatgcagtg gtccacaaag tggtagcctg gtaacttgac acccttctcc
      181 ttagctttgt aagtagttct gaagtcacat cggtaatggg catttccttc aagcaacaaa
      241 gccatatgaa tatcacccgt cagcactcca tcacgcacat acattttctc agtggagggc
      301 tcccatttca gcgtcttctt ctgcataact ggaccattgg cgggaaagtt ggtaccataa
      361 aatcgaactt tattatagaa agtgtcccct tccattgtta tgtcgtttct ggcaatgcaa
      421 atgcccccgt cttcgaaagt caagcttcgt tcccacgaat accccttagg aaacgactgc
      481 ttaaaatagt cttgtatgtt gtctggatat ttggcgaata ccctgttgcc gtaatggaat
      541 gcagtggtca ggatatcaaa ggcaaaaggc agaggtccgc cctctttgac ttcaagatcc
      601 atactctgtt ttccctcaaa aggcttgcct gtaccatctc cgtcgatcac aaagtggtgc
      661 ccgtttacgt tgccttccat acggagtttg atcttcatgt ctggcttaat cgcactcatg
      721 gatccccatc gatccttatc gtcatcgtcg tacagatccc gacccatttg ctgtccacca
      781 gtcatgctag ccataccatg atgatgatga tgatgagaac cccgcatatg tatatctcct
      841 tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt gtggtctccc tatagtgagt
      901 cgtattaatt tcgcgggatc gagatcctgc attaatgaat cggccaacgc gcggggagag
      961 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1021 ttcggctgcg gcgag
//
