LOCUS Exported 6060 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pcDNA5/FRT/TO DNAJB7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6060) AUTHORS Hageman J, Kampinga HH TITLE Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library. JOURNAL Cell Stress Chaperones. 2008 Aug 7. ():. PUBMED 18686016 REFERENCE 2 (bases 1 to 6060) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6060 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 820..838 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 841..859 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 869..893 /label=LNCX /note="Human CMV promoter, forward primer" primer_bind complement(2012..2029) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2018..2242 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(2375..2394) /label=F1ori-R /note="F1 origin, reverse primer" protein_bind 2526..2573 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS 2581..3603 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 3733..3854 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3770..3789) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3824..3843 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3903..3919) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3903..3919) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3916..3938) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3927..3943 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3951..3981) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3996..4017 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4134..4151) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4305..4893) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4385..4404) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5064..5924) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5687..5706 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5925..6029) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga 1021 attctgcaga tatcaccatg gtggattact atgaagttct aggactgcaa agatatgctt 1081 cacctgagga cattaaaaaa gcttatcata aagtggcact taaatggcac cctgataaaa 1141 atccagaaaa taaagaagaa gcagagagaa aattcaaaga agtagctgag gcatacgagg 1201 tattatcaaa tgatgagaaa cgggacattt atgataaata tggcacagaa ggattaaacg 1261 gaggtggaag tcattttgat gatgaatgtg agtacggctt cacattccat aagccagatg 1321 atgtttttaa agaaattttt catgaaaggg atccattttc ttttcacttc tttgaagact 1381 cgcttgagga cctgttaaat cgtccaggaa gctcctatgg aaacagaaac agagatgcag 1441 gatacttttt ctccactgcc agtgaatatc caatttttga gaaattttct tcatatgata 1501 caggatatac atcacagggt tcattggggc atgaaggcct tacttctttc tcttccctgg 1561 cttttgataa tagtgggatg gacaactaca tatctgttac aacttcagac aaaatcgtta 1621 atggcagaaa tattaataca aagaaaatta ttgaaagtga tcaagaaaga gaagctgaag 1681 ataatggaga gttgacattt tttcttgtaa atagtgtggc caatgaagag ggctttgcaa 1741 aagaatgcag ctggagaaca cagtcattca acaactattc accaaattct cacagctcca 1801 aacatgtatc tcaatatact ttcgtggaca atgatgaggg aggtatatct tgggttacca 1861 gcaacagaga tccccctatt ttctcagcag gagtcaaaga gggtggtaag aggaaaaaaa 1921 agaagcgtaa agaggtgcaa aagaagtcta ccaaaaggaa ttgttaagcg gccgctcgag 1981 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca 2041 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 2101 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 2161 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 2221 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg 2281 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 2341 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 2401 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 2461 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 2521 acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc 2581 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc 2641 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta 2701 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt 2761 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg 2821 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa 2881 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg 2941 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc 3001 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac 3061 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg 3121 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc 3181 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg 3241 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt 3301 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg 3361 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc 3421 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc 3481 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt 3541 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa 3601 tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 3661 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 3721 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 3781 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 3841 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca 3901 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 3961 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 4021 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 4081 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 4141 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 4201 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 4261 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 4321 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 4381 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 4441 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 4501 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 4561 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 4621 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 4681 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 4741 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 4801 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 4861 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 4921 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 4981 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 5041 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 5101 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 5161 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 5221 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 5281 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 5341 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 5401 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 5461 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 5521 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 5581 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 5641 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 5701 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 5761 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 5821 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 5881 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 5941 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 6001 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc //