LOCUS Exported 5801 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pcDNA5/FRT/TO DNAJB9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5801) AUTHORS Hageman J, Kampinga HH TITLE Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library. JOURNAL Cell Stress Chaperones. 2008 Aug 7. ():. PUBMED 18686016 REFERENCE 2 (bases 1 to 5801) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5801 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 820..838 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 841..859 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 869..893 /label=LNCX /note="Human CMV promoter, forward primer" primer_bind complement(1753..1770) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1759..1983 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(2116..2135) /label=F1ori-R /note="F1 origin, reverse primer" protein_bind 2267..2314 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS 2322..3344 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 3474..3595 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3511..3530) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3565..3584 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3644..3660) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3644..3660) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3657..3679) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3668..3684 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3692..3722) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3737..3758 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3875..3892) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4046..4634) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4126..4145) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4805..5665) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5428..5447 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5666..5770) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga 1021 attctgcaga tatccagcac agtggcggcc gcaccatggc tactccccag tcaattttca 1081 tctttgcaat ctgcatttta atgataacag aattaattct ggcctcaaaa agctactatg 1141 atatcttagg tgtgccaaaa tcggcatcag agcgccaaat caagaaggcc tttcacaagt 1201 tggccatgaa gtaccaccct gacaaaaata agagcccgga tgctgaagca aaattcagag 1261 agattgcaga agcatatgaa acactctcag atgctaatag acgaaaagag tatgatacac 1321 ttggacacag tgcttttact agtggtaaag gacaaagagg tagtggaagt tcttttgagc 1381 agtcatttaa cttcaatttt gatgacttat ttaaagactt tggctttttt ggtcaaaacc 1441 aaaacactgg atccaagaag cgttttgaaa atcatttcca gacacgccag gatggtggtt 1501 ccagtagaca aaggcatcat ttccaagaat tttcttttgg aggtggatta tttgatgaca 1561 tgtttgaaga tatggagaaa atgttttctt ttagtggttt tgactctacc aatcagcata 1621 cagtacagac tgaaaataga tttcatggat ctagcaagca ctgcaggact gtcactcaac 1681 gaagaggaaa tatggttact acatacactg actgttcagg acagtagggg cccgtttaaa 1741 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 1801 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 1861 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 1921 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 1981 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta 2041 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 2101 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 2161 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 2221 acctcgaccc caaaaaactt gattagggtg atggttcacg tacctagaag ttcctattcc 2281 gaagttccta ttctctagaa agtataggaa cttccttggc caaaaagcct gaactcaccg 2341 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc 2401 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc 2461 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg 2521 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga 2581 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac 2641 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta 2701 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc 2761 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg 2821 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact 2881 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca 2941 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg 3001 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct 3061 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc 3121 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt 3181 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac 3241 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata 3301 gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgta ctacgagatt 3361 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 3421 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt 3481 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 3541 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 3601 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 3661 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 3721 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 3781 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 3841 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 3901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 3961 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 4021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 4081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 4141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 4201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 4261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 4321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 4381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 4441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 4501 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 4561 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 4621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 4681 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 4741 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 4801 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 4861 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 4921 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 4981 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 5041 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 5101 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 5161 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 5221 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 5281 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 5341 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 5401 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 5461 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 5521 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 5581 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 5641 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 5701 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 5761 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c //