LOCUS       Exported                 998 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  Strong constitutive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    PlepA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     96..113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      137..194
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    195..215
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    373..394
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      397..440
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(444..462)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(690..709)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
       61 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct
      121 gcataacgcg aagtaatctt ttcggtttta aagaaaaagg gcagggtggt gacaccttgc
      181 ccttttttgc cggactgcag cggccgctac tagtaactat taaacgcaaa atacactagc
      241 ttagattata tcaataggat tgtaaagatt caatgtaaaa caagaaagag aaaagttccc
      301 tatcatatct gggttgcttt tctctttcac gtacttattg attcatatcc cgtatccatt
      361 catacattga ctctctagaa gcggccgcga attccagaaa tcatccttag cgaaagctaa
      421 ggattttttt tatctgaaat tctgcctcgt gatacgccta tttttatagg ttaatgtcat
      481 gataataatg gtttcttaga cgtcaggtgg cactcgagtt gatcgggcac gtaagaggtt
      541 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      601 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      661 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      721 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      781 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      841 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      901 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      961 tccggcagtt tctacacata tattcgcaag atgtggcg
//
