LOCUS Exported 16336 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF. ACCESSION . VERSION . KEYWORDS T7-VEE-OKS-iM SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 16336) AUTHORS Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF TITLE Efficient generation of human iPSCs by a synthetic self-replicative RNA. JOURNAL Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001. PUBMED 23910086 REFERENCE 2 (bases 1 to 16336) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..16336 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 1535..1543 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" regulatory 7586..7595 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 8678..8731 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 10154..10213 /codon_start=1 /product="2A peptide from equine rhinitis A virus polyprotein" /label=E2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="QCTNYALLKLAGDVESNPGP" regulatory 11812..11821 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 11818..13140 /codon_start=1 /product="human c-Myc proto-oncogene" /label=c-Myc /translation="MGPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPA PSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVT ELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSP NPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSD SLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESG SPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNN RKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKK ATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA" CDS 13777..14376 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(13777..13796) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 14273..14293 /label=Puro-F /note="Puromycin resistance gene, forward primer" promoter 14574..14678 /gene="bla" /label=AmpR promoter CDS 14679..15539 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(14897..14916) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 15710..16298 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 16199..16218 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 16319..16336 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 atgggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg 61 ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg 121 aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc 181 tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa 241 gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat 301 gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg 361 aaataactga taaggaattg gacaagaaaa tgaaggagct ggccgccgtc atgagcgacc 421 ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc 481 aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag 541 ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta 601 agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa 661 cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt 721 ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga 781 ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact 841 tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg 901 tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta 961 cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg 1021 tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac 1081 tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta 1141 tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg 1201 tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa 1261 ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc 1321 acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg 1381 atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa 1441 caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg 1501 acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt 1561 tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaagccgatg 1621 tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa 1681 aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg 1741 ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga 1801 taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg 1861 tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca 1921 ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag 1981 gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg 2041 aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag 2101 ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa 2161 cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag 2221 gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga 2281 aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg 2341 ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata 2401 ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac 2461 ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc 2521 tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc 2581 gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa 2641 cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc 2701 aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca 2761 aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg 2821 ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg 2881 tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga 2941 taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag 3001 cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc 3061 agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca 3121 tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact 3181 cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg 3241 gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc 3301 cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc 3361 cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc 3421 gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag 3481 tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg 3541 gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt 3601 tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg 3661 tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc 3721 agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc 3781 tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa 3841 gcatcattgg tgctatagcg cggcagttca agttttcccg ggtatgcaaa ccgaaatcct 3901 cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc 3961 acaattctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg 4021 aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag 4081 gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc 4141 tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac 4201 tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt 4261 cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca 4321 acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga 4381 acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg 4441 cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg 4501 ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg 4561 atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca 4621 caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg 4681 atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca 4741 tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg 4801 aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa 4861 gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat 4921 tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct 4981 caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag 5041 acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac 5101 cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg 5161 aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg 5221 aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat 5281 ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca 5341 gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc 5401 gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa 5461 gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc 5521 caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc 5581 ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga 5641 ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg 5701 catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa 5761 cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc 5821 tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta 5881 acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta 5941 ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc 6001 tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg 6061 cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta 6121 ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca 6181 ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac 6241 ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag 6301 ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg 6361 cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt 6421 ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa 6481 aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca 6541 taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa 6601 aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag 6661 cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga 6721 acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact 6781 tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg 6841 acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt 6901 tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta 6961 aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag 7021 tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg 7081 cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag 7141 acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga 7201 aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc 7261 gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg 7321 aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg 7381 gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca 7441 tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag 7501 gggcccctat aactctctac ggctaacctg aatggactac gacatagtct agtccgccaa 7561 gtctagcata tgggcgcgtg aattcgccac catggcggga cacctggctt cggatttcgc 7621 cttctcgccc cctccaggtg gtggaggtga tgggccaggg gggccggagc cgggctgggt 7681 tgatcctcgg acctggctaa gcttccaagg ccctcctgga gggccaggaa tcgggccggg 7741 ggttgggcca ggctctgagg tgtgggggat tcccccatgc cccccgccgt atgagttctg 7801 tggggggatg gcgtactgtg ggccccaggt tggagtgggg ctagtgcccc aaggcggctt 7861 ggagacctct cagcctgagg gcgaagcagg agtcggggtg gagagcaact ccgatggggc 7921 ctccccggag ccctgcaccg tcacccctgg tgccgtgaag ctggagaagg agaagctgga 7981 gcaaaacccg gaggagtccc aggacatcaa agctctgcag aaagaactcg agcaatttgc 8041 caagctcctg aagcagaaga ggatcaccct gggatataca caggccgatg tggggctcac 8101 cctgggggtt ctatttggga aggtattcag ccaaacgacc atctgccgct ttgaggctct 8161 gcagcttagc ttcaagaaca tgtgtaagct gcggcccttg ctgcagaagt gggtggagga 8221 agctgacaac aatgaaaatc ttcaggagat atgcaaagca gaaaccctcg tgcaggcccg 8281 aaagagaaag cgaaccagta tcgagaaccg agtgagaggc aacctggaga atttgttcct 8341 gcagtgcccg aaacccacac tgcagcagat cagccacatc gcccagcagc ttgggctcga 8401 gaaggatgtg gtccgagtgt ggttctgtaa ccggcgccag aagggcaagc gatcaagcag 8461 cgactatgca caacgagagg attttgaggc tgctgggtct cctttctcag ggggaccagt 8521 gtcctttcct ctggccccag ggccccattt tggtacccca ggctatggga gccctcactt 8581 cactgcactg tactcctcgg tccctttccc tgagggggaa gcctttcccc ctgtctccgt 8641 caccactctg ggctctccca tgcattcaaa ctctagtgag ggcagaggaa gtctgctaac 8701 atgcggtgac gtcgaggaga atcctggccc acaattgatg gctgtcagcg acgcgctgct 8761 cccatctttc tccacgttcg cgtctggccc ggcgggaagg gagaagacac tgcgtcaagc 8821 aggtgccccg aataaccgct ggcgggagga gctctcccac atgaagcgac ttcccccagt 8881 gcttcccggc cgcccctatg acctggcggc ggcgaccgtg gccacagacc tggagagcgg 8941 cggagccggt gcggcttgcg gcggtagcaa cctggcgccc ctacctcgga gagagaccga 9001 ggagttcaac gatctcctgg acctggactt tattctctcc aattcgctga cccatcctcc 9061 ggagtcagtg gccgccaccg tgtcctcgtc agcgtcagcc tcctcttcgt cgtcgccgtc 9121 gagcagcggc cctgccagcg cgccctccac ctgcagcttc acctatccga tccgggccgg 9181 gaacgacccg ggcgtggcgc cgggcggcac gggcggaggc ctcctctatg gcagggagtc 9241 cgctccccct ccgacggctc ccttcaacct ggcggacatc aacgacgtga gcccctcggg 9301 cggcttcgtg gccgagctcc tgcggccaga attggacccg gtgtacattc cgccgcagca 9361 gccgcagccg ccaggtggcg ggctgatggg caagttcgtg ctgaaggcgt cgctgagcgc 9421 ccctggcagc gagtacggca gcccgtcggt catcagcgtc agcaaaggca gccctgacgg 9481 cagccacccg gtggtggtgg cgccctacaa cggcgggccg ccgcgcacgt gccccaagat 9541 caagcaggag gcggtctctt cgtgcaccca cttgggcgct ggaccccctc tcagcaatgg 9601 ccaccggccg gctgcacacg acttccccct ggggcggcag ctccccagca ggactacccc 9661 gaccctgggt cttgaggaag tgctgagcag cagggactgt caccctgccc tgccgcttcc 9721 tcccggcttc catccccacc cggggcccaa ttacccatcc ttcctgcccg atcagatgca 9781 gccgcaagtc ccgccgctcc attaccaaga gctcatgcca cccggttcct gcatgccaga 9841 ggagcccaag ccaaagaggg gaagacgatc gtggccccgg aaaaggaccg ccacccacac 9901 ttgtgattac gcgggctgcg gcaaaaccta cacaaagagt tcccatctca aggcacacct 9961 gcgaacccac acaggtgaga aaccttacca ctgtgactgg gacggctgtg gatggaaatt 10021 cgcccgctca gatgaactga ccaggcacta ccgtaaacac acggggcacc gcccgttcca 10081 gtgccaaaaa tgcgaccgag cattttccag gtcggaccac ctcgccttac acatgaagag 10141 gcatttttct agacaatgta ctaactacgc tttgttgaaa ctcgctggcg atgttgaaag 10201 taaccccggt cctggcgcgc ccatgtacaa catgatggag acggagctga agccgccggg 10261 cccgcagcaa acttcggggg gcggcggcgg caactccacc gcggcggcgg ccggcggcaa 10321 ccagaaaaac agcccggacc gcgtcaagcg gcccatgaat gccttcatgg tgtggtcccg 10381 cgggcagcgg cgcaagatgg cccaggagaa ccccaagatg cacaactcgg agatcagcaa 10441 gcgcctgggc gccgagtgga aacttttgtc ggagacggag aagcggccgt tcatcgacga 10501 ggctaagcgg ctgcgagcgc tgcacatgaa ggagcacccg gattataaat accggccccg 10561 gcggaaaacc aagacgctca tgaagaagga taagtacacg ctgcccggcg ggctgctggc 10621 ccccggcggc aatagcatgg cgagcggggt cggggtgggc gccggcctgg gcgcgggcgt 10681 gaaccagcgc atggacagtt acgcgcacat gaacggctgg agcaacggca gctacagcat 10741 gatgcaggac cagctgggct acccgcagca cccgggcctc aatgcgcacg gcgcagcgca 10801 gatgcagccc atgcaccgct acgacgtgag cgccctgcag tacaactcca tgaccagctc 10861 gcagacctac atgaacggct cgcccaccta cagcatgtcc tactcgcagc agggcacccc 10921 tggcatggct cttggctcca tgggttcggt ggtcaagtcc gaggccagct ccagcccccc 10981 tgtggttacc tcttcctccc actccagggc gccctgccag gccggggacc tccgggacat 11041 gatcagcatg tatctccccg gcgccgaggt gccggaaccc gccgccccca gcagacttca 11101 catgtcccag cactaccaga gcggcccggt gcccggcacg gccattaacg gcacactgcc 11161 cctctcacac atgtgagcgg ccatcgatgt cgacaactaa cttaagctag caacggtttc 11221 cctctagcgg gatcaattcc gccccccccc cctaacgtta ctggccgaag ccgcttggaa 11281 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat 11341 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct 11401 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct 11461 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc 11521 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa 11581 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc 11641 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg 11701 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc 11761 ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccaatt cgccaccatg 11821 ggccccctca acgttagctt caccaacagg aactatgacc tcgactacga ctcggtgcag 11881 ccgtatttct actgcgacga ggaggagaac ttctaccagc agcagcagca gagcgagctg 11941 cagcccccgg cgcccagcga ggatatctgg aagaaattcg agctgctgcc caccccgccc 12001 ctgtccccta gccgccgctc cgggctctgc tcgccctcct acgttgcggt cacacccttc 12061 tcccttcggg gagacaacga cggcggtggc gggagcttct ccacggccga ccagctggag 12121 atggtgaccg agctgctggg aggagacatg gtgaaccaga gtttcatctg cgacccggac 12181 gacgagacct tcatcaaaaa catcatcatc caggactgta tgtggagcgg cttctcggcc 12241 gccgccaagc tcgtctcaga gaagctggcc tcctaccagg ctgcgcgcaa agacagcggc 12301 agcccgaacc ccgcccgcgg ccacagcgtc tgctccacct ccagcttgta cctgcaggat 12361 ctgagcgccg ccgcctcaga gtgcatcgac ccctcggtgg tcttccccta ccctctcaac 12421 gacagcagct cgcccaagtc ctgcgcctcg caagactcca gcgccttctc tccgtcctcg 12481 gattctctgc tctcctcgac ggagtcctcc ccgcagggca gccccgagcc cctggtgctc 12541 catgaggaga caccgcccac caccagcagc gactctgagg aggaacaaga agatgaggaa 12601 gaaatcgatg ttgtttctgt ggaaaagagg caggctcctg gcaaaaggtc agagtctgga 12661 tcaccttctg ctggaggcca cagcaaacct cctcacagcc cactggtcct caagaggtgc 12721 cacgtctcca cacatcagca caactacgca gcgcctccct ccactcggaa ggactatcct 12781 gctgccaaga gggtcaagtt ggacagtgtc agagtcctga gacagatcag caacaaccga 12841 aaatgcacca gccccaggtc ctcggacacc gaggagaatg tcaagaggcg aacacacaac 12901 gtcttggagc gccagaggag gaacgagcta aaacggagct tttttgccct gcgtgaccag 12961 atcccggagt tggaaaacaa tgaaaaggcc cccaaggtag ttatccttaa aaaagccaca 13021 gcatacatcc tgtccgtcca agcagaggag caaaagctca tttctgaaga ggacttgttg 13081 cggaaacgac gagaacagtt gaaacacaaa cttgaacagc tacggaactc ttgtgcgtaa 13141 tctagagtcg acccgggcgg ccgcaactaa cttaagctag caacggtttc cctctagcgg 13201 gatcaattcc gccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt 13261 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc 13321 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag 13381 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac 13441 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc 13501 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc 13561 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca 13621 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt 13681 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg 13741 gggacgtggt tttcctttga aaaacacgat aataccatga ccgagtacaa gcccacggtg 13801 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 13861 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 13921 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 13981 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 14041 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 14101 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 14161 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 14221 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 14281 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 14341 cgcacctggt gcatgacccg caagcccggt gcctgagaat tggcaagctg cttacataga 14401 actcgcggcg attggcatgc cgccttaaaa tttttatttt atttttcttt tcttttccga 14461 atcggatttt gtttttaata tttcaaaaaa aaaaaaaaaa aaaaaaaaaa cgcgtcgagg 14521 ggaattaatt cttgaagacg aaagggccag gtggcacttt tcggggaaat gtgcgcggaa 14581 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 14641 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 14701 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 14761 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 14821 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 14881 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc 14941 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 15001 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 15061 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 15121 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 15181 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 15241 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 15301 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 15361 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 15421 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 15481 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 15541 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 15601 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 15661 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 15721 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 15781 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 15841 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 15901 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 15961 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 16021 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 16081 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg 16141 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 16201 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 16261 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcgagctcta 16321 atacgactca ctatag //